Molecular Role of EGFR-MAPK Pathway in Patchouli Alcohol-Induced Apoptosis and Cell Cycle Arrest on A549 Cells and

Overview

Journal Biomed Res Int

Publisher Wiley

Specialties Biomedical Engineering
Biotechnology
General Medicine

Date 2016 Nov 11

PMID 27830146

Citations 17

Authors

Xingang Lu

Liu Yang

Chenghua Lu

Zhenyu Xu

Hongfu Qiu

Jiajia Wu

Jingwen Wang

Jiafeng Tong

Yin Zhu

Jie Shen

Affiliations

Soon will be listed here.

Abstract

Nowadays, chemotherapy is still the main effective treatment for cancer. Herb prescriptions containing (also known as "Guang-Huo-Xiang") have been widely used in Chinese medicine today. In our research, we found that patchouli alcohol, a compound isolated from the oil of , exerted antitumor ability against human lung cancer A549 cells ability both and . MTT assay was used to assess cell viability. Hoechst 33342 staining and TUNEL cover glass staining provided the visual evidence of apoptosis. Caspase activity measurement showed that patchouli alcohol activated caspase 9 and caspase 3 of mitochondria-mediated apoptosis. Consistently, patchouli alcohol inhibited the xenograft tumor . Further investigation of the underlying molecular mechanism showed that MAPK and EGFR pathway might contribute to the antitumor effect of patchouli alcohol. Our study proved that patchouli alcohol might be able to serve as a novel antitumor compound in the clinical treatment of lung cancer.

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