Integrative Omics Connects N-glycoproteome-wide Alterations with Pathways and Regulatory Events in Induced Pluripotent Stem Cells

Overview

Journal Sci Rep

Specialty Science

Date 2016 Nov 4

PMID 27808266

Citations 1

Authors

Putty-Reddy Sudhir

Madireddy Pavana Kumari

Wei-Ting Hsu

Chein-Hung Chen

Hung-Chih Kuo

Chung-Hsuan Chen

Affiliations

Soon will be listed here.

Abstract

Molecular-level differences ranging from genomes to proteomes, but not N-glycoproteomes, between human induced pluripotent stem cells (hiPSCs) and embryonic stem cells (hESCs) have been assessed to gain insights into cell reprogramming and induced pluripotency. Our multiplexed quantitative N-glycoproteomics study identified altered N-glycoproteins that significantly regulate cell adhesion processes in hiPSCs compared to hESCs. The integrative proteomics and functional network analyses of the altered N-glycoproteins revealed their significant interactions with known PluriNet (pluripotency-associated network) proteins. We found that these interactions potentially regulate various signaling pathways including focal adhesion, PI3K-Akt signaling, regulation of actin cytoskeleton, and spliceosome. Furthermore, the integrative transcriptomics analysis revealed that imperfectly reprogrammed subunits of the oligosaccharyltransferase (OST) and dolichol-phosphate-mannose synthase (DPM) complexes were potential candidate regulatory events for the altered N-glycoprotein levels. Together, the results of our study suggest that imperfect reprogramming of the protein complexes linked with the N-glycosylation process may result in N-glycoprotein alterations that affect induced pluripotency through their functional protein interactions.

Citing Articles

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