How Many Papillomavirus Species Can Go Undetected in Papilloma Lesions?

Overview

Journal Sci Rep

Specialty Science

Date 2016 Nov 4

PMID 27808255

Citations 20

Authors

Cintia Daudt

Flavio R C da Silva

Andre F Streck

Matheus N Weber

Fabiana Q Mayer

Samuel P Cibulski

Claudio W Canal

Affiliations

Soon will be listed here.

Abstract

A co-infection comprising to at least seven papillomavirus (PV) types was detected by next generation sequencing (NGS) of randomly primed rolling circle amplification (RCA) products of a bovine (Bos taurus) papilloma lesion from the Brazilian Amazon region. Six putative new PV types that could not be detected by commonly used PCR protocols were identified. Their overall L1 nucleotide identities were less than 90% compared to described PV species and types. L1 nucleotide BLAST sequence hits showed that each new type was related to Beta, Gamma, Dyokappa, Dyoeta, and Xipapillomavirus, as well as two likely new unclassified genera. Our results show that the employment of NGS is relevant to the detection and characterization of distantly related PV and is of major importance in co-infection studies. This knowledge will help us understand the biology and pathogenesis of PV, as well as contribute to disease control. Moreover, we can also conclude that there are many unknown circulating PVs.

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