Microbiological Challenge Testing for in Ready-to-Eat Food: A Practical Approach

Overview

Journal Ital J Food Saf

Publisher PagePress

Date 2014 Dec 10

PMID 27800369

Citations 7

Authors

Carlo Spanu

Christian Scarano

Michela Ibba

Carlo Pala

Vincenzo Spanu

Enrico Pietro Luigi De Santis

Affiliations

Soon will be listed here.

Abstract

Food business operators (FBOs) are the primary responsible for the safety of food they place on the market. The definition and validation of the product's shelf-life is an essential part for ensuring microbiological safety of food and health of consumers. In the frame of the Regulation (EC) No 2073/2005 on microbiological criteria for foodstuffs, FBOs shall conduct shelf-life studies in order to assure that their food does not exceed the food safety criteria throughout the defined shelf-life. In particular this is required for ready-to-eat (RTE) food that supports the growth of . Among other studies, FBOs can rely on the conclusion drawn by microbiological challenge tests. A microbiological challenge test consists in the artificial contamination of a food with a pathogen microorganism and aims at simulating its behaviour during processing and distribution under the foreseen storage and handling conditions. A number of documents published by international health authorities and research institutions describes how to conduct challenge studies. The authors reviewed the existing literature and described the methodology for implementing such laboratory studies. All the main aspects for the conduction of microbiological challenge tests were considered, from the selection of the strains, preparation and choice of the inoculum level and method of contamination, to the experimental design and data interpretation. The objective of the present document is to provide an exhaustive and practical guideline for laboratories that want to implement challenge testing on RTE food.

Citing Articles

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Effects of High-Pressure Processing, UV-C Irradiation and Thermoultrasonication on Donor Human Milk Safety and Quality.

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References

Knight T, Castillo A, Maxim J, Keeton J, Miller R . Effectiveness of potassium lactate and sodium diacetate in combination with irradiation to control Listeria monocytogenes on frankfurters. J Food Sci. 2007; 72(1):M026-30. DOI: 10.1111/j.1750-3841.2006.00221.x. View

Konteles S, Sinanoglou V, Batrinou A, Sflomos K . Effects of gamma-irradiation on Listeria monocytogenes population, colour, texture and sensory properties of Feta cheese during cold storage. Food Microbiol. 2009; 26(2):157-65. DOI: 10.1016/j.fm.2008.10.006. View

Guyer S, Jemmi T . Behavior of Listeria monocytogenes during fabrication and storage of experimentally contaminated smoked salmon. Appl Environ Microbiol. 1991; 57(5):1523-7. PMC: 182979. DOI: 10.1128/aem.57.5.1523-1527.1991. View

Morales P, Calzada J, Nunez M . Effect of high-pressure treatment on the survival of Listeria monocytogenes Scott A in sliced vacuum-packaged Iberian and Serrano cured hams. J Food Prot. 2006; 69(10):2539-43. DOI: 10.4315/0362-028x-69.10.2539. View

Selby T, Berzins A, Gerrard D, Corvalan C, Grant A, Linton R . Microbial heat resistance of Listeria monocytogenes and the impact on ready-to-eat meat quality after post-package pasteurization. Meat Sci. 2011; 74(3):425-34. DOI: 10.1016/j.meatsci.2006.02.018. View

Foong S, Gonzalez G, Dickson J . Reduction and survival of Listeria monocytogenes in ready-to-eat meats after irradiation. J Food Prot. 2004; 67(1):77-82. DOI: 10.4315/0362-028x-67.1.77. View

Carminati D, Gatti M, Bonvini B, Neviani E, Mucchetti G . High-pressure processing of Gorgonzola cheese: influence on Listeria monocytogenes inactivation and on sensory characteristics. J Food Prot. 2004; 67(8):1671-5. DOI: 10.4315/0362-028x-67.8.1671. View

McCormick K, Han I, Acton J, Sheldon B, Dawson P . D and z-values for Listeria monocytogenes and Salmonella typhimurium in packaged low-fat ready-to-eat turkey bologna subjected to a surface pasteurization treatment. Poult Sci. 2003; 82(8):1337-42. DOI: 10.1093/ps/82.8.1337. View

Marcos B, Aymerich T, Monfort J, Garriga M . High-pressure processing and antimicrobial biodegradable packaging to control Listeria monocytogenes during storage of cooked ham. Food Microbiol. 2007; 25(1):177-82. DOI: 10.1016/j.fm.2007.05.002. View

10.

Lucore L, Shellhammer T, Yousef A . Inactivation of Listeria monocytogenes Scott A on artificially contaminated frankfurters by high-pressure processing. J Food Prot. 2000; 63(5):662-4. DOI: 10.4315/0362-028x-63.5.662. View

11.

Peck M . Clostridium botulinum and the safety of minimally heated, chilled foods: an emerging issue?. J Appl Microbiol. 2006; 101(3):556-70. DOI: 10.1111/j.1365-2672.2006.02987.x. View

12.

Corry J, Jarvis B, Hedges A . Minimising the between-sample variance in colony counts on foods. Food Microbiol. 2010; 27(5):598-603. DOI: 10.1016/j.fm.2010.02.002. View

13.

Hereu A, Bover-Cid S, Garriga M, Aymerich T . High hydrostatic pressure and biopreservation of dry-cured ham to meet the Food Safety Objectives for Listeria monocytogenes. Int J Food Microbiol. 2011; 154(3):107-12. DOI: 10.1016/j.ijfoodmicro.2011.02.027. View

14.

Jin T, Liu L, Sommers C, Boyd G, Zhang H . Radiation sensitization and postirradiation proliferation of Listeria monocytogenes on ready-to-eat deli meat in the presence of pectin-nisin films. J Food Prot. 2009; 72(3):644-9. DOI: 10.4315/0362-028x-72.3.644. View

15.

Murphy R, Duncan L, Driscoll K, Marcy J, Beard B . Thermal inactivation of Listeria monocytogenes on ready-to-eat turkey breast meat products during postcook in-package pasteurization with hot water. J Food Prot. 2003; 66(9):1618-22. DOI: 10.4315/0362-028x-66.9.1618. View

16.

Muriana P, Quimby W, Davidson C, Grooms J . Postpackage pasteurization of ready-to-eat deli meats by submersion heating for reduction of Listeria monocytogenes. J Food Prot. 2002; 65(6):963-9. DOI: 10.4315/0362-028x-65.6.963. View

17.

Augustin J, Bergis H, Midelet-Bourdin G, Cornu M, Couvert O, Denis C . Design of challenge testing experiments to assess the variability of Listeria monocytogenes growth in foods. Food Microbiol. 2011; 28(4):746-54. DOI: 10.1016/j.fm.2010.05.028. View

18.

Zhu M, Du M, Cordray J, Ahn D . Control of Listeria monocytogenes Contamination in Ready-to-Eat Meat Products. Compr Rev Food Sci Food Saf. 2021; 4(2):34-42. DOI: 10.1111/j.1541-4337.2005.tb00071.x. View

19.

Skalina L, Nikolajeva V . Growth potential of Listeria monocytogenes strains in mixed ready-to-eat salads. Int J Food Microbiol. 2010; 144(2):317-21. DOI: 10.1016/j.ijfoodmicro.2010.10.001. View

20.

Wu V, Fung D, Kang D, Thompson L . Evaluation of thin agar layer method for recovery of acid-injured foodborne pathogens. J Food Prot. 2001; 64(7):1067-71. DOI: 10.4315/0362-028x-64.7.1067. View