Does 24bp Duplication of Human Gene (Chitotriosidase1) Predispose to Filarial Chyluria? A Case-Control Study

Overview

Journal J Clin Diagn Res

Specialty General Medicine

Date 2016 Oct 30

PMID 27790506

Citations 2

Authors

Shriya Pant

Jyotsna Agarwal

Apul Goel

Pravin K Gangwar

Mohammad Waseem

Prashant Gupta

Satya N Sankhwar

Bimalesh Purkait

Affiliations

Soon will be listed here.

Abstract

Introduction: Chyluria which is endemic in many parts of the world is mainly caused by (chitotriosidase) is produced by macrophages and plays an important role in the defense against chitin containing pathogen such as filarial parasite. Variation in the coding region with 24 bp duplication allele results in reduced activity that enhance the survival of parasite which may play a role in the occurrence of disease.

Aim: To examine the role of 24bp duplication of gene in patients of filarial chyluria (FC).

Materials And Methods: A case-control study was carried out where 155 confirmed FC patients and equal number of age-, sex- and residence-matched controls without any symptoms or signs of lymphatic filariasis, confirmed by negative immunochromatographic card test (ICT) and IgG/IgM combo rapid antibody test, from a hospital-based population were enrolled. Filarial aetiology was confirmed on the basis of DEC-provocative test (Giemsa staining), ICT and IgG/IgM- antifiarial antibody test. The patients positive by either of these tests were enrolled as FC cases. 24bp duplication in gene in FC was detected by the product size 99bp of amplified gene using polymerase chain reaction.

Results: The mean ages of patients and controls were 38.25±12.09 and 35.45±12.53 years, respectively while male: female ratio was 2.4:1. The mean duration of illness in chyluria patients was 62.81±60.83 months and mean number of episodes was 2.54±1.11. Homozygous wild type, heterozygous and homozygous mutant frequencies were 10.3%, 81.3% and 8.4% in FC patients and 18.7%, 75.5%, and 5.8% in controls, respectively. The 24bp duplication in gene showed a significant association in Heterozygous (HT) genotype with Odd Ratio (OR) of 1.95, 95% Confidence Interval (CI) (1.01-3.77); p=0.04. However, the homozygous mutant genotype (TT) was found to be non-significant with OR of 2.61, 95% CI (0.91-7.45); p=0.07. The combination of both HT+TT was also found to be significant with OR of 2.00, 95% CI (1.03-3.85); p=0.03.

Conclusion: In this study from Northern India, gene polymorphism showed an influence as a possible risk factor for susceptibility to FC. Further studies need to be done on a larger number of FC patients in different regions of the country.

Citing Articles

Chitotriosidase: a marker and modulator of lung disease.

Chang D, Sharma L, Dela Cruz C Eur Respir Rev. 2020; 29(156).

PMID: 32350087 PMC: 9488994. DOI: 10.1183/16000617.0143-2019.

The Genetic Polymorphisms of 24 Base Pair Duplication and Point G102S of Human Chitotriosidase to Bancroftian Filariasis at the Thai⁻Myanmar Border.

Sanprasert V, Charuchaibovorn S, Nuchprayoon S Pathogens. 2019; 8(1).

PMID: 30934653 PMC: 6470825. DOI: 10.3390/pathogens8010041.

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