Evaluating Frequency and Quality of Pathogen-specific T Cells

Overview

Journal Nat Commun

Specialty Biology

Date 2016 Oct 28

PMID 27786275

Citations 7

Authors

Nadia Anikeeva

Dolores Grosso

Neal Flomenberg

Yuri Sykulev

Affiliations

Soon will be listed here.

Abstract

It is generally accepted that enumeration and characterization of antigen-specific T cells provide essential information about potency of the immune response. Here, we report a new technique to determine the frequency and potency of antigen-specific CD8 T cells. The assay measures changes of intracellular Ca in real time by fluorescent microscopy in individual CD8 T cells responding to cognate peptides. The T cells form continuous monolayer, enabling the cells to present the peptides to each other. This approach allows us to evaluate the kinetics of intracellular Ca signalling that characterizes the quality of T cell response. We demonstrate the usefulness of the assay examining the frequency and quality of cytomegalovirus-specific CD8 T cells from healthy donor and patient after haploidentical stem cell transplantation. The new assay has a potential to provide essential information determining the status of the immune system, disease morbidity, potency of therapeutic intervention and vaccine efficacy.

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