Association of the C677T (rs1801133) Polymorphism with Idiopathic Male Infertility in a Local Pakistani Population

Overview

Journal Balkan J Med Genet

Publisher Sciendo

Specialty Genetics

Date 2016 Oct 28

PMID 27785408

Citations 9

Authors

M Irfan

M Ismail

M Azhar Beg

A Shabbir

A Rashid Kayani

G Kaukab Raja

Affiliations

Soon will be listed here.

Abstract

The present study determined an association between idiopathic sperm disorders in a local Pakistani infertile male population and the C677T polymorphism. After ruling out non genetic factors, a total of 437 idiopathic infertile men including 57 azoospermic, 66 oligospermic, 44 asthenozoospermic, 29 teratozoospermic, 20 oligoasthenospermic and 221 infertile normospermic men were recruited. Furthermore, 218 normospermic fertile men, who had two children (or more) were included as controls. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was used to determine C677T (rs1801133) polymorphism. A significant association of the minor 677T allele with male infertility was observed <0.05). In addition, men with 677 CT and TT genotypes were at a greater risk [odds ratio (OR): 1.81, 95% confidence interval (95% CI): 1.17-2.80, = 0.008 and OR: 9.24, 95% CI: 1.20-70.92, = 0.032, respectively] of infertility. All the subgroups of male infertility (azoospermic, oligospermic, asthenospermic, oligoasthenoteratospermic (OAT) and normospermic infertile) had significantly <0.05) higher frequencies of CT and TT genotypes when compared to fertile men. The combined genotypes (CT + TT) were also found significantly (OR: 2.01, 95% CI: 1.31-3.08, <0.001) associated with male infertility. The results suggest that the polymorphism might be a factor of male infertility in the Pakistani population.

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