Pentraxin 3 (PTX3) Promoter Methylation Associated with PTX3 Plasma Levels and Neutrophil to Lymphocyte Ratio in Coronary Artery Disease

Overview

Journal J Geriatr Cardiol

Specialty Geriatrics

Date 2016 Oct 27

PMID 27781062

Citations 8

Authors

Tang-Meng Guo

Li-Li Huang

Kai Liu

Li Ke

Zhi-Jian Luo

Yun-Qiao Li

Xing-Lin Chen

Bei Cheng

Affiliations

Soon will be listed here.

Abstract

Background: Pentraxin 3 (PTX3) is expressed in the heart under inflammatory conditions and plays an important role in atherogenesis. Patients with increased PTX3 levels may suffer from higher rates of cardiac events. Regulation of specific genes by promoter methylation is important in atherogenesis. The factors influencing PTX3 levels and the association between epigenetics and PTX3 levels have not been investigated.

Methods: Blood samples were collected from 64 patients admitted to the Department of Cardiology, 35 who had coronary artery disease (CAD), and 29 who were CAD-free. Plasma levels of PTX3 were measured by ELISA. PTX3 promoter methylation was evaluated via methyl-specific PCR. The severity of coronary artery lesion was evaluated by angiography.

Results: The level of PTX3 promoter methylation in the CAD group was 62.69% ± 20.57%, significantly lower than that of the CAD-free group, which was 72.45% ± 11.84% ( = 0.03). Lower PTX3 promoter methylation levels in the CAD group were associated with higher plasma PTX3 concentrations ( = -0.29, = 0.02). Furthermore, lower PTX3 promoter methylation levels were associated with higher neutrophil to lymphocyte ratio (NLR) in men ( = -0.58, = 0.002).

Conclusions: The present study provides new evidence that methylation of the PTX3 promoter is associated with PTX3 plasma levels and NLR in coronary artery disease. This study also shows that modification of epigenetics by chronic inflammation might be a significant molecular mechanism in the atherosclerotic processes that influence plasma PTX3 concentrations.

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