» Articles » PMID: 27779216

Soluble P-selectin Rescues Viper Venom-induced Mortality Through Anti-inflammatory Properties and PSGL-1 Pathway-mediated Correction of Hemostasis

Overview
Journal Sci Rep
Specialty Science
Date 2016 Oct 26
PMID 27779216
Citations 19
Authors
Affiliations
Soon will be listed here.
Abstract

Venomous snakebites are lethal and occur frequently worldwide each year, and receiving the antivenom antibody is currently the most effective treatment. However, the specific antivenom might be unavailable in remote areas. Snakebites by Viperidae usually lead to hemorrhage and mortality if untreated. In the present study, challenges of rattlesnake (Crotalus atrox) venom markedly increased the circulating soluble P-selectin (sP-sel) level, but not P-selectin (P-sel, Selp) mutants, in wild-type mice. Because sP-sel enhances coagulation through the P-selectin ligand 1 (PSGL-1, Selplg) pathway to produce tissue factor-positive microparticles, we hypothesized that increasing the plasma sP-sel level can be a self-rescue response in hosts against snake venom-mediated suppression of the coagulation system. Confirming our hypothesis, our results indicated that compared with wild-type mice, Selp and Selplg mice were more sensitive to rattlesnake venom. Additionally, administration of recombinant sP-sel could effectively reduce the mortality rate of mice challenged with venoms from three other Viperidae snakes. The antivenom property of sP-sel is associated with improved coagulation activity in vivo. Our data suggest that the elevation of endogenous sP-sel level is a self-protective response against venom-suppressed coagulation. The administration of recombinant sP-sel may be developed as a new strategy to treat Viperidae snakebites.

Citing Articles

Targeted Delivery to Dying Cells Through P-Selectin-PSGL-1 Axis: A Promising Strategy for Enhanced Drug Efficacy in Liver Injury Models.

Lien T, Sun D, Chang H Cells. 2024; 13(21.

PMID: 39513885 PMC: 11545035. DOI: 10.3390/cells13211778.


Platelet-derived circulating soluble P-selectin is sufficient to induce hematopoietic stem cell mobilization.

Wang T, Liou Y, Yang S, Lin G, Chiang Y, Lien T Stem Cell Res Ther. 2023; 14(1):300.

PMID: 37864264 PMC: 10589967. DOI: 10.1186/s13287-023-03527-w.


Restraint Stress-Induced Immunosuppression Is Associated with Concurrent Macrophage Pyroptosis Cell Death in Mice.

Li C, Munalisa R, Lee H, Lien T, Chan H, Hung S Int J Mol Sci. 2023; 24(16).

PMID: 37629059 PMC: 10454201. DOI: 10.3390/ijms241612877.


Dual phenotypic characteristics of P-selectin in a mouse model of hemorrhagic shock and hepatectomy.

Chen J, Cheng T, Huang C, Chang H, Lam C Heliyon. 2023; 9(8):e18627.

PMID: 37554775 PMC: 10404689. DOI: 10.1016/j.heliyon.2023.e18627.


The Role of Activating Transcription Factor 3 in Metformin's Alleviation of Gastrointestinal Injury Induced by Restraint Stress in Mice.

Siwakoti B, Lien T, Lin Y, Pethaperumal S, Hung S, Sun D Int J Mol Sci. 2023; 24(13).

PMID: 37446172 PMC: 10342009. DOI: 10.3390/ijms241310995.


References
1.
Chang H, Shyu H, Sun D, Shyu R, Tang S . Facilitation of cell adhesion by immobilized dengue viral nonstructural protein 1 (NS1): arginine-glycine-aspartic acid structural mimicry within the dengue viral NS1 antigen. J Infect Dis. 2002; 186(6):743-51. DOI: 10.1086/342600. View

2.
Chang H, Kau J, Lo S, Sun D . Cell-adhesion and morphological changes are not sufficient to support anchorage-dependent cell growth via non-integrin-mediated attachment. Cell Biol Int. 2003; 27(2):123-33. DOI: 10.1016/s1065-6995(02)00295-0. View

3.
McEver R . Adhesive interactions of leukocytes, platelets, and the vessel wall during hemostasis and inflammation. Thromb Haemost. 2001; 86(3):746-56. View

4.
Chang H, Hu S, Huang T, Chen S, Lee Y, Lo S . Rhodostomin, an RGD-containing peptide expressed from a synthetic gene in Escherichia coli, facilitates the attachment of human hepatoma cells. Biochem Biophys Res Commun. 1993; 190(1):242-9. DOI: 10.1006/bbrc.1993.1037. View

5.
Michels M, van der Ven A, Djamiatun K, Fijnheer R, de Groot P, Griffioen A . Imbalance of angiopoietin-1 and angiopoetin-2 in severe dengue and relationship with thrombocytopenia, endothelial activation, and vascular stability. Am J Trop Med Hyg. 2012; 87(5):943-6. PMC: 3516273. DOI: 10.4269/ajtmh.2012.12-0020. View