Label-free Detection of MicroRNA Based on Coupling Multiple Isothermal Amplification Techniques

Overview

Journal Sci Rep

Specialty Science

Date 2016 Oct 26

PMID 27777399

Citations 3

Authors

Xiangjiang Zheng

Li Niu

Di Wei

Xuemei Li

Shusheng Zhang

Affiliations

Soon will be listed here.

Abstract

MicroRNA (miRNA) was a promising class of cancer biomarkers. Here we developed a label-free method for sensitive measurement of let-7d miRNA based on multiple amplification techniques. The primer will bind to the duplex strand DNA that was formed by stem-loop template and target let-7d to initiate strand displacement amplification (SDA) in tandem. The released single strand DNA will be a primer to bind the circular template to initiate rolling circle amplification (RCA). The products based on multiple amplifications will be detected by a standard fluorimeter with N-methyl mesoporphyrin IX (NMM) as the fluorescent indicator. The proposed method exhibited excellent selectivity and high sensitivity with a detection limit of as low as 1.5 × 10 M. Moreover, this methodology was used for the determination of biomolecules in real serum samples with satisfying results.

Citing Articles

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