Immunoelectron Microscopy of Lipopolysaccharide in Chlamydia Trachomatis

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1989 Oct 1

PMID 2777384

Citations 7

Authors

S Birkelund

A G Lundemose

G Christiansen

Affiliations

Soon will be listed here.

Abstract

Monoclonal antibodies (MAb) specific for Chlamydia trachomatis lipopolysaccharide (LPS) and major outer membrane protein (MOMP) were used for immunoelectron microscopy analysis. MAb specific for MOMP showed strong reaction with the chlamydial surface, whereas MAb specific for LPS showed strong association of gold particles with the periphery of the chlamydial body. After fixation of the chlamydia cells, the reactivity was, however, similar to the anti-MOMP reactivity. Enzyme-linked immunosorbent assay showed that MAb specific for LPS could remove LPS from the chlamydial membrane.

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References

Taylor H, PRENDERGAST R . Attempted oral immunization with chlamydial lipopolysaccharide subunit vaccine. Invest Ophthalmol Vis Sci. 1987; 28(10):1722-6. View

Birkelund S, Lundemose A, Christiansen G . Chemical cross-linking of Chlamydia trachomatis. Infect Immun. 1988; 56(3):654-9. PMC: 259341. DOI: 10.1128/iai.56.3.654-659.1988. View

Robinson Jr E, McGee Z, Buchanan T, Blake M, Hitchcock P . Probing the surface of Neisseria gonorrhoeae: simultaneous localization of protein I and H.8 antigens. Infect Immun. 1987; 55(5):1190-7. PMC: 260489. DOI: 10.1128/iai.55.5.1190-1197.1987. View

Brade H, Brade L, Nano F . Chemical and serological investigations on the genus-specific lipopolysaccharide epitope of Chlamydia. Proc Natl Acad Sci U S A. 1987; 84(8):2508-12. PMC: 304683. DOI: 10.1073/pnas.84.8.2508. View

Zhang Y, Stewart S, Joseph T, Taylor H, Caldwell H . Protective monoclonal antibodies recognize epitopes located on the major outer membrane protein of Chlamydia trachomatis. J Immunol. 1987; 138(2):575-81. View

Caldwell H, Stewart S, Johnson S, Taylor H . Tear and serum antibody response to Chlamydia trachomatis antigens during acute chlamydial conjunctivitis in monkeys as determined by immunoblotting. Infect Immun. 1987; 55(1):93-8. PMC: 260284. DOI: 10.1128/iai.55.1.93-98.1987. View

Behnke O, Ammitzboll T, Jessen H, Klokker M, NILAUSEN K, Tranum-Jensen J . Non-specific binding of protein-stabilized gold sols as a source of error in immunocytochemistry. Eur J Cell Biol. 1986; 41(2):326-38. View

Joiner K, Grossman N, Schmetz M, Leive L . C3 binds preferentially to long-chain lipopolysaccharide during alternative pathway activation by Salmonella montevideo. J Immunol. 1986; 136(2):710-5. View

Nano F, Caldwell H . Expression of the chlamydial genus-specific lipopolysaccharide epitope in Escherichia coli. Science. 1985; 228(4700):742-4. DOI: 10.1126/science.2581315. View

10.

Robinson Jr E, McGee Z, Kaplan J, Hammond M, Larson J, Buchanan T . Ultrastructural localization of specific gonococcal macromolecules with antibody-gold sphere immunological probes. Infect Immun. 1984; 46(2):361-6. PMC: 261539. DOI: 10.1128/iai.46.2.361-366.1984. View

11.

Stephens R, Tam M, Kuo C, Nowinski R . Monoclonal antibodies to Chlamydia trachomatis: antibody specificities and antigen characterization. J Immunol. 1982; 128(3):1083-9. View

12.

Wang S, GRAYSTON J . Human serology in Chlamydia trachomatis infection with microimmunofluorescence. J Infect Dis. 1974; 130(4):388-97. DOI: 10.1093/infdis/130.4.388. View

13.

Kuo C, Chi E . Ultrastructural study of Chlamydia trachomatis surface antigens by immunogold staining with monoclonal antibodies. Infect Immun. 1987; 55(5):1324-8. PMC: 260509. DOI: 10.1128/iai.55.5.1324-1328.1987. View

14.

Flynn P, Shenep J, Gigliotti F, Davis D, Hildner W . Immunolabeling of lipopolysaccharide liberated from antibiotic-treated Escherichia coli. Infect Immun. 1988; 56(10):2760-2. PMC: 259641. DOI: 10.1128/iai.56.10.2760-2762.1988. View

15.

Rank R, Batteiger B, Soderberg L . Susceptibility to reinfection after a primary chlamydial genital infection. Infect Immun. 1988; 56(9):2243-9. PMC: 259556. DOI: 10.1128/iai.56.9.2243-2249.1988. View

16.

Fuerst J, Perry J . Demonstration of lipopolysaccharide on sheathed flagella of Vibrio cholerae O:1 by protein A-gold immunoelectron microscopy. J Bacteriol. 1988; 170(4):1488-94. PMC: 210992. DOI: 10.1128/jb.170.4.1488-1494.1988. View

17.

Hackstadt T . Steric hindrance of antibody binding to surface proteins of Coxiella burnetti by phase I lipopolysaccharide. Infect Immun. 1988; 56(4):802-7. PMC: 259373. DOI: 10.1128/iai.56.4.802-807.1988. View

18.

Robinson Jr E, Clemens C, McGee Z, Cannon J . Immunoelectron microscopic localization of outer membrane proteins II on the surface of Neisseria gonorrhoeae. Infect Immun. 1988; 56(4):1003-6. PMC: 259407. DOI: 10.1128/iai.56.4.1003-1006.1988. View

19.

Katz B, Batteiger B, Jones R . Effect of prior sexually transmitted disease on the isolation of Chlamydia trachomatis. Sex Transm Dis. 1987; 14(3):160-4. DOI: 10.1097/00007435-198707000-00008. View

20.

Frank M, Joiner K, Hammer C . The function of antibody and complement in the lysis of bacteria. Rev Infect Dis. 1987; 9 Suppl 5:S537-45. DOI: 10.1093/clinids/9.supplement_5.s537. View