Characterization of the Macrophage Subset Affected and Its Response to a T Suppressor Factor (TsFmp) Found in Cryptococcosis

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1989 Oct 1

PMID 2777368

Citations 2

Authors

R Blackstock

N C Hernandez

Affiliations

Soon will be listed here.

Abstract

Previous reports from our laboratory described the detection of a suppressor factor which inhibited the phagocytic activity of a macrophage subset in murine cryptococcosis and in classical models of immune tolerance. The suppressor factor was originally named PIL (phagocytosis-inhibiting lymphokine) but has recently been renamed TsFmp (T suppressor factor for macrophage phagocytosis) because it was found to resemble the antigen-specific I-J-restricted suppressor factors described by others. The current investigation revealed that TsFmp acted rapidly upon the macrophage (15 min or less) to exert its effect of inhibiting the phagocytic process. The time for the macrophage to recover from the effects of TsFmp was likewise very rapid. The ability of TsFmp to inhibit phagocytosis was limited to engulfment of particles by Fc and mannan receptors and did not extent to phagocytosis via complement receptors or by nonspecific mechanisms. The macrophage subset that responded to TsFmp was determined to be in the I-A+ and I-J-IM+ subset.

Citing Articles

Immunological aspects of fungal pathogenesis.

Deepe G, Bullock W Eur J Clin Microbiol Infect Dis. 1990; 9(8):567-79.

PMID: 2209625 DOI: 10.1007/BF01967211.

Immunity to a pulmonary Cryptococcus neoformans infection requires both CD4+ and CD8+ T cells.

Huffnagle G, Yates J, Lipscomb M J Exp Med. 1991; 173(4):793-800.

PMID: 1672543 PMC: 2190813. DOI: 10.1084/jem.173.4.793.

References

Ptak W, Zembala M, Asherson G . Nonspecific macrophage suppressor factor: its role in the inhibition of contact sensitivity to picryl chloride by specific T suppressor factor. Eur J Immunol. 1978; 8(9):645-9. DOI: 10.1002/eji.1830080908. View

Blackstock R, Hall N, Hernandez N . Characterization of a suppressor factor that regulates phagocytosis by macrophages in murine cryptococcosis. Infect Immun. 1989; 57(6):1773-9. PMC: 313355. DOI: 10.1128/iai.57.6.1773-1779.1989. View

Zembala M, Asherson G, JAMES B, Stein V, Watkins M . Anti-haptene T suppressor factor acts through an I-J+, Ly1-2+, T acceptor cell that releases a nonspecific inhibitor of the transfer of contact sensitivity when exposed to antigen. J Immunol. 1982; 129(5):1823-9. View

Malkovsky M, Asherson G, Chandler P, Colizzi V, Watkins M, Zembala M . Nonspecific inhibitor of DNA synthesis elaborated by T acceptor cells. I. Specific hapten- and I-J-driven liberation of an inhibitor of cell proliferation by Lyt-1-2+ cyclophosphamide-sensitive T acceptor cells armed with a product of.... J Immunol. 1983; 130(2):785-90. View

Morgan M, Blackstock R, Bulmer G, Hall N . Modification of macrophage phagocytosis in murine cryptococcosis. Infect Immun. 1983; 40(2):493-500. PMC: 264882. DOI: 10.1128/iai.40.2.493-500.1983. View

Murphy J, Mosley R, Moorhead J . Regulation of cell-mediated immunity in cryptococcosis. II. Characterization of first-order T suppressor cells (Ts1) and induction of second-order suppressor cells. J Immunol. 1983; 130(6):2876-81. View

Jardieu P, Uede T, Ishizaka K . IgE-binding factors from mouse T lymphocytes. III. Role of antigen-specific suppressor T cells in the formation of IgE-suppressive factor. J Immunol. 1984; 133(6):3266-73. View

Murphy J . Effects of first-order Cryptococcus-specific T-suppressor cells on induction of cells responsible for delayed-type hypersensitivity. Infect Immun. 1985; 48(2):439-45. PMC: 261339. DOI: 10.1128/iai.48.2.439-445.1985. View

Sung S, Nelson R, Silverstein S . Mouse peritoneal macrophages plated on mannan- and horseradish peroxidase-coated substrates lose the ability to phagocytose by their Fc receptors. J Immunol. 1985; 134(6):3712-7. View

10.

Dorf M, Benacerraf B . Suppressor cells and immunoregulation. Annu Rev Immunol. 1984; 2:127-57. DOI: 10.1146/annurev.iy.02.040184.001015. View

11.

Kawasaki H, Martin C, Uchida T, Usui M, Noma T, Minami M . Functional analysis of cloned macrophage hybridomas. V. Induction of suppressor T cell responses. J Immunol. 1986; 137(7):2145-51. View

12.

Blackstock R, McCormack J, Hall N . Induction of a macrophage-suppressive lymphokine by soluble cryptococcal antigens and its association with models of immunologic tolerance. Infect Immun. 1987; 55(1):233-9. PMC: 260308. DOI: 10.1128/iai.55.1.233-239.1987. View

13.

Khakpour F, Murphy J . Characterization of a third-order suppressor T cell (Ts3) induced by cryptococcal antigen(s). Infect Immun. 1987; 55(7):1657-62. PMC: 260574. DOI: 10.1128/iai.55.7.1657-1662.1987. View

14.

Jaureguiberry B, Liao L, Kuchroo V, Dorf M, Diamond B . Monoclonal anti-idiotypic antibodies to an I-J interacting molecule inhibit suppression in an H-2 restricted way. J Immunol. 1988; 140(10):3286-9. View

15.

Blackstock R, Hernandez N . Inhibition of macrophage phagocytosis in cryptococcosis: phenotypic analysis of the suppressor cell. Cell Immunol. 1988; 114(1):174-87. DOI: 10.1016/0008-8749(88)90264-x. View

16.

Steele J, Kuchroo V, Kawasaki H, Jayaraman S, Iwata M, Ishizaka K . A monoclonal antibody raised to lipomodulin recognizes T suppressor factors in two independent hapten-specific suppressor networks. J Immunol. 1989; 142(7):2213-20. View

17.

Murphy J, Moorhead J . Regulation of cell-mediated immunity in cryptococcosis. I. Induction of specific afferent T suppressor cells by cryptococcal antigen. J Immunol. 1982; 128(1):276-83. View