Rapid Scan Electron Paramagnetic Resonance Opens New Avenues for Imaging Physiologically Important Parameters In Vivo

Overview

Journal J Vis Exp

Specialties Biology
General Medicine

Date 2016 Oct 22

PMID 27768025

Citations 7

Authors

Joshua R Biller

Deborah G Mitchell

Mark Tseytlin

Hanan Elajaili

George A Rinard

Richard W Quine

Sandra S Eaton

Gareth R Eaton

Affiliations

Soon will be listed here.

Abstract

We demonstrate a superior method of 2D spectral-spatial imaging of stable radical reporter molecules at 250 MHz using rapid-scan electron-paramagnetic-resonance (RS-EPR), which can provide quantitative information under in vivo conditions on oxygen concentration, pH, redox status and concentration of signaling molecules (i.e., OH, NO). The RS-EPR technique has a higher sensitivity, improved spatial resolution (1 mm), and shorter acquisition time in comparison to the standard continuous wave (CW) technique. A variety of phantom configurations have been tested, with spatial resolution varying from 1 to 6 mm, and spectral width of the reporter molecules ranging from 16 µT (160 mG) to 5 mT (50 G). A cross-loop bimodal resonator decouples excitation and detection, reducing the noise, while the rapid scan effect allows more power to be input to the spin system before saturation, increasing the EPR signal. This leads to a substantially higher signal-to-noise ratio than in conventional CW EPR experiments.

Citing Articles

4D-image reconstruction directly from limited-angular-range data in continuous-wave electron paramagnetic resonance imaging.

Zhang Z, Epel B, Chen B, Xia D, Sidky E, Qiao Z J Magn Reson. 2023; 350:107432.

PMID: 37058955 PMC: 10197356. DOI: 10.1016/j.jmr.2023.107432.

Rapid scan EPR: Automated digital resonator control for low-latency data acquisition.

OConnell R, Tseytlin O, Bobko A, Eubank T, Tseytlin M J Magn Reson. 2022; 345:107308.

PMID: 36356489 PMC: 10266206. DOI: 10.1016/j.jmr.2022.107308.

Rapid Scan EPR imaging as a Tool for Magnetic Field Mapping.

Tseytlin O, Bobko A, Tseytlin M Appl Magn Reson. 2021; 51(9-10):1117-1124.

PMID: 33642700 PMC: 7909464. DOI: 10.1007/s00723-020-01238-6.

EPR Everywhere.

Biller J, McPeak J Appl Magn Reson. 2021; 52(8):1113-1139.

PMID: 33519097 PMC: 7826499. DOI: 10.1007/s00723-020-01304-z.

Rapid-scan EPR imaging of a phantom comprised of species with different linewidths and relaxation times.

Shi Y, Eaton S, Eaton G J Magn Reson. 2019; 308():106593.

PMID: 31520789 PMC: 6829054. DOI: 10.1016/j.jmr.2019.106593.

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