Immunohistochemistry of Aldosterone Synthase Leads the Way to the Pathogenesis of Primary Aldosteronism

Overview

Journal Mol Cell Endocrinol

Specialties Cell Biology
Endocrinology
Molecular Biology

Date 2016 Oct 25

PMID 27751767

Citations 27

Authors

Koshiro Nishimoto

Minae Koga

Tsugio Seki

Kenji Oki

Elise P Gomez-Sanchez

Celso E Gomez-Sanchez

Mitsuhide Naruse

Tomokazu Sakaguchi

Shinya Morita

Takeo Kosaka

Mototsugu Oya

Tadashi Ogishima

Masanori Yasuda

Makoto Suematsu

Yasuaki Kabe

Masao Omura

Tetsuo Nishikawa

Kuniaki Mukai

Affiliations

Soon will be listed here.

Abstract

Our group previously purified human and rat aldosterone synthase (CYP11B2 and Cyp11b2, respectively) from their adrenals and verified that it is distinct from steroid 11β-hydroxylase (CYP11B1 or Cyp11b1), the cortisol- or corticosterone-synthesizing enzyme. We now describe their distributions immunohistochemically with specific antibodies. In rats, there is layered functional zonation with the Cyp11b2-positive zona glomerulosa (ZG), Cyp11b1-positive zona fasciculata (ZF), and Cyp11b2/Cyp11b1-negative undifferentiated zone between the ZG and ZF. In human infants and children (<12 years old), the functional zonation is similar to that in rats. In adults, the adrenal cortex remodels and subcapsular aldosterone-producing cell clusters (APCCs) replace the continuous ZG layer. We recently reported possible APCC-to-APA transitional lesions (pAATLs) in 2 cases of unilateral multiple adrenocortical micro-nodules. In this review, we present 4 additional cases of primary aldosteronism, from which the extracted adrenals contain pAATLs, with results of next generation sequencing for these lesions. Immunohistochemistry for CYP11B2 and CYP11B1 has become an important tool for the diagnosis of and research on adrenocortical pathological conditions and suggests that APCCs may be the origin of aldosterone-producing adenoma.

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