Amnioserosa Cell Constriction but Not Epidermal Actin Cable Tension Autonomously Drives Dorsal Closure

Overview

Journal Nat Cell Biol

Specialty Cell Biology

Date 2016 Oct 28

PMID 27749821

Citations 40

Authors

Laurynas Pasakarnis

Erich Frei

Emmanuel Caussinus

Markus Affolter

Damian Brunner

Affiliations

Soon will be listed here.

Abstract

Tissue morphogenesis requires coordination of multiple force-producing components. During dorsal closure in fly embryogenesis, an epidermis opening closes. A tensioned epidermal actin/MyosinII cable, which surrounds the opening, produces a force that is thought to combine with another MyosinII force mediating apical constriction of the amnioserosa cells that fill the opening. A model proposing that each force could autonomously drive dorsal closure was recently challenged by a model in which the two forces combine in a ratchet mechanism. Acute force elimination via selective MyosinII depletion in one or the other tissue shows that the amnioserosa tissue autonomously drives dorsal closure while the actin/MyosinII cable cannot. These findings exclude both previous models, although a contribution of the ratchet mechanism at dorsal closure onset remains likely. This shifts the current view of dorsal closure being a combinatorial force-component system to a single tissue-driven closure event.

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