Immunohistochemical Localization of HTLV-I P19- and P24-related Antigens in Synovial Joints of Patients with Rheumatoid Arthritis

Overview

Journal Am J Pathol

Publisher Elsevier

Specialty Pathology

Date 1989 Jul 1

PMID 2774054

Citations 12

Authors

B Ziegler

R E Gay

G Q Huang

H G Fassbender

S Gay

Affiliations

Soon will be listed here.

Abstract

In formalin-fixed, paraffin-embedded synovial tissues from patients with early proliferative rheumatoid arthritis (RA), immunoreactivity could be demonstrated utilizing monoclonal IgG antibodies reactive with the p19 and p24 protein of human T cell leukemia virus (HTLV-I). Additionally, surgical specimens of fresh unfixed synovial tissues from patients with RA also demonstrated immunoreactivity. At the light microscopic level, both HTLV-I antigens were detected in approximately 45% of the rheumatoid synovial tissues by the immunocolloidal gold method with silver enhancement (IGSS) and the avidin-biotin-complex technique (ABC), whereas six of eight of the frozen RA specimens stained positive by immunofluorescence. Patients whose synovial tissues were immunoreactive by immunofluorescence were seronegative to HTLV-I antigens as determined by ELISA and immunoblotting. Conversely, cases with osteoarthritis, juvenile rheumatoid arthritis, psoriatic arthritis, Dupuytren's contracture, and gangrene were shown to be nonreactive by immunohistochemistry. The results indicate that expression of antigens is related to or crossreactive with HTLV-I in synovial tissues from patients with rheumatoid arthritis.

Citing Articles

Role of Viral Infections in the Pathogenesis of Sjögren's Syndrome: Different Characteristics of Epstein-Barr Virus and HTLV-1.

Nakamura H, Shimizu T, Kawakami A J Clin Med. 2020; 9(5).

PMID: 32414149 PMC: 7290771. DOI: 10.3390/jcm9051459.

Human Endogenous Retroviruses (HERVs) and Autoimmune Rheumatic Disease: Is There a Link?.

Tugnet N, Rylance P, Roden D, Trela M, Nelson P Open Rheumatol J. 2013; 7:13-21.

PMID: 23750183 PMC: 3636489. DOI: 10.2174/1874312901307010013.

Demystified. Human endogenous retroviruses.

Nelson P, Carnegie P, Martin J, Ejtehadi H, Hooley P, Roden D Mol Pathol. 2003; 56(1):11-8.

PMID: 12560456 PMC: 1187282. DOI: 10.1136/mp.56.1.11.

Human endogenous retroviruses: nature, occurrence, and clinical implications in human disease.

Urnovitz H, MURPHY W Clin Microbiol Rev. 1996; 9(1):72-99.

PMID: 8665478 PMC: 172883. DOI: 10.1128/CMR.9.1.72.

Molecular and cellular mechanisms of joint destruction in rheumatoid arthritis: two cellular mechanisms explain joint destruction?.

Gay S, Gay R, Koopman W Ann Rheum Dis. 1993; 52 Suppl 1:S39-47.

PMID: 8481058 PMC: 1035025. DOI: 10.1136/ard.52.suppl_1.s39.

References

Miyoshi I, Kubonishi I, Yoshimoto S, Akagi T, Ohtsuki Y, Shiraishi Y . Type C virus particles in a cord T-cell line derived by co-cultivating normal human cord leukocytes and human leukaemic T cells. Nature. 1981; 294(5843):770-1. DOI: 10.1038/294770a0. View

Pantazis P, Sariban E, Bohan C, Antoniades H, Kalyanaraman V . Synthesis of PDGF by cultured human T cells transformed with HTLV-I and II. Oncogene. 1987; 1(3):285-9. View

Blattner W, Kalyanaraman V, Robert-Guroff M, Lister T, GALTON D, SARIN P . The human type-C retrovirus, HTLV, in Blacks from the Caribbean region, and relationship to adult T-cell leukemia/lymphoma. Int J Cancer. 1982; 30(3):257-64. DOI: 10.1002/ijc.2910300302. View

Popovic M, SARIN P, Kalyanaraman V, Mann D, Minowada J, Gallo R . Isolation and transmission of human retrovirus (human t-cell leukemia virus). Science. 1983; 219(4586):856-9. DOI: 10.1126/science.6600519. View

Haynes B, Robert-Guroff M, METZGAR R, Franchini G, Kalyanaraman V, Palker T . Monoclonal antibody against human T cell leukemia virus p19 defines a human thymic epithelial antigen acquired during ontogeny. J Exp Med. 1983; 157(3):907-20. PMC: 2186953. DOI: 10.1084/jem.157.3.907. View

Fassbender H . Histomorphological basis of articular cartilage destruction in rheumatoid arthritis. Coll Relat Res. 1983; 3(2):141-55. DOI: 10.1016/s0174-173x(83)80040-5. View

Seiki M, Hattori S, Hirayama Y, Yoshida M . Human adult T-cell leukemia virus: complete nucleotide sequence of the provirus genome integrated in leukemia cell DNA. Proc Natl Acad Sci U S A. 1983; 80(12):3618-22. PMC: 394101. DOI: 10.1073/pnas.80.12.3618. View

Suni J, Narvanen A, Wahlstrom T, Lehtovirta P, Vaheri A . Monoclonal antibody to human T-cell leukemia virus p19 defines polypeptide antigen in human choriocarcinoma cells and syncytiotrophoblasts of first-trimester placentas. Int J Cancer. 1984; 33(3):293-8. DOI: 10.1002/ijc.2910330303. View

Palker T, Scearce R, Miller S, Popovic M, Bolognesi D, Gallo R . Monoclonal antibodies against human T cell leukemia-lymphoma virus (HTLV) p24 internal core protein. Use as diagnostic probes and cellular localization of HTLV. J Exp Med. 1984; 159(4):1117-31. PMC: 2187274. DOI: 10.1084/jem.159.4.1117. View

10.

Schumacher Jr H . Synovial membrane and fluid morphologic alterations in early rheumatoid arthritis: microvascular injury and virus-like particles. Ann N Y Acad Sci. 1975; 256:39-64. DOI: 10.1111/j.1749-6632.1975.tb36034.x. View

11.

Yoshikura H, Nishida J, Yoshida M, Kitamura Y, Takaku F, Ikeda S . Isolation of HTLV derived from Japanese adult T-cell leukemia patients in human diploid fibroblast strain IMR90 and the biological characters of the infected cells. Int J Cancer. 1984; 33(6):745-9. DOI: 10.1002/ijc.2910330606. View

12.

Ho D, ROTA T, Hirsch M . Infection of human endothelial cells by human T-lymphotropic virus type I. Proc Natl Acad Sci U S A. 1984; 81(23):7588-90. PMC: 392192. DOI: 10.1073/pnas.81.23.7588. View

13.

Hoxie J, Matthews D, Cines D . Infection of human endothelial cells by human T-cell leukemia virus type I. Proc Natl Acad Sci U S A. 1984; 81(23):7591-5. PMC: 392193. DOI: 10.1073/pnas.81.23.7591. View

14.

Shaw G, Broder S, Essex M, Gallo R . Human T-cell leukemia virus: its discovery and role in leukemogenesis and immunosuppression. Adv Intern Med. 1984; 30:1-27. View

15.

Weiss R, Clapham P, Nagy K, Hoshino H . Envelope properties of human T-cell leukemia viruses. Curr Top Microbiol Immunol. 1985; 115:235-46. DOI: 10.1007/978-3-642-70113-9_15. View

16.

Palker T, Bolognesi D, Haynes B . Human T-cell leukemia/lymphoma virus: studies of host-virus interaction. Curr Top Microbiol Immunol. 1985; 115:247-66. DOI: 10.1007/978-3-642-70113-9_16. View

17.

Palker T, Scearce R, Ho W, Copeland T, Oroszlan S, Popovic M . Monoclonal antibodies reactive with human T cell lymphotropic virusI (HTLVI) p19 internal core protein: cross-reactivity with normal tissues and differential reactivity with HTLV types I and II. J Immunol. 1985; 135(1):247-54. View

18.

Narayan O, Sheffer D, Clements J, Tennekoon G . Restricted replication of lentiviruses. Visna viruses induce a unique interferon during interaction between lymphocytes and infected macrophages. J Exp Med. 1985; 162(6):1954-69. PMC: 2187990. DOI: 10.1084/jem.162.6.1954. View

19.

Panayi G, Dalgleish A . Retroviruses in rheumatoid arthritis. Ann Rheum Dis. 1986; 45(5):439. PMC: 1001911. DOI: 10.1136/ard.45.5.439-b. View

20.

Phillips P, Johnston S, Runge L, Moore J, Poiesz B . High IgM antibody to human T-lymphotropic virus type I in systemic lupus erythematosus. J Clin Immunol. 1986; 6(3):234-41. DOI: 10.1007/BF00918703. View