Transport of Corilagin, Gallic Acid, and Ellagic Acid from Fructus Phyllanthi Tannin Fraction in Caco-2 Cell Monolayers

Overview

Journal Evid Based Complement Alternat Med

Specialty Rehabilitation Medicine

Date 2016 Oct 15

PMID 27738446

Citations 13

Authors

Xin Mao

Ling-Fang Wu

Hai-Juan Zhao

Wen-Yi Liang

Wen-Jing Chen

Shu-Xian Han

Qi Qi

Ya-Ping Cui

Shi Li

Guang-Hui Yang

Yan-Yan Shao

Dan Zhu

Ru-Feng Wang

Yun You

Lan-Zhen Zhang

Affiliations

Soon will be listed here.

Abstract

. To investigate the absorption property of the representative hydrolyzable tannin, namely corilagin, and its hydrolysates gallic acid (GA) and ellagic acid (EA) from the Fructus Phyllanthi tannin fraction (PTF) . . Caco-2 cells monolayer model was established. Influences of PTF on Caco-2 cells viability were detected with MTT assay. The transport across monolayers was examined for different time points, concentrations, and secretory directions. The inhibitors of P-glycoprotein (P-gp), multidrug resistance proteins (MRPs), organic anion transporting polypeptide (OATP) and sodium/glucose cotransporter 1 (SGLT1), and tight junction modulators were used to study the transport mechanism. LC-MS method was employed to quantify the absorption concentration. . The apparent permeability coefficient () values of the three compounds were below 1.0 × 10 cm/s. The absorption of corilagin and GA were much lower than their efflux, and the uptake of both compounds was increased in the presence of inhibitors of P-gp and MRPs. The absorption of EA was decreased in the company of OATP and SGLT1 inhibitors. Moreover, the transport of corilagin, GA, and EA was enhanced by tight junction modulators. . These observations indicated that the three compounds in PTF were transported via passive diffusion combined with protein mediated transport. P-gp and MRPs might get involved in the transport of corilagin and GA. The absorption of EA could be attributed to OATP and SGLT1 protein.

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