Characterization and in Vitro Sensitivity of Cholinesterases of Gilthead Seabream (Sparus Aurata) to Organophosphate Pesticides

Overview

Journal Fish Physiol Biochem

Specialty Biochemistry

Date 2016 Oct 8

PMID 27714546

Citations 2

Authors

G Albendin

J M Arellano

M P Manuel-Vez

C Sarasquete

M I Arufe

Affiliations

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Abstract

The characterization of cholinesterase activity in brain and muscle of gilthead seabream was carried out using four specific substrates and three selective inhibitors. In addition, K and V were calculated from the Michaelis-Menten equation for ASCh and BSCh substrates. Finally, the in vitro sensitivity of brain and muscle cholinesterases to three organophosphates (OPs) was also investigated by estimating inhibition kinetics. The results indicate that AChE is the enzyme present in the brain, whereas in muscle, a typical AChE form is present along with an atypical form of BChE. Very low ChE activity was found in plasma with all substrates used. The inhibitory potency of the studied OPs on brain and muscle AChEs based on bimolecular inhibition constants (k ) was: omethoate < dichlorvos < azinphosmethyl-oxon. Furthermore, muscle BChE was found to be several orders of magnitude (from 2 to 4) more sensitive than brain and muscle AChE inhibition by dichlorvos and omethoate.

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References

Silva Filho M, Oliveira M, Salles J, Cunha Bastos V, Cassano V, Bastos J . Methyl-paraoxon comparative inhibition kinetics for acetylcholinesterases from brain of neotropical fishes. Toxicol Lett. 2004; 153(2):247-54. DOI: 10.1016/j.toxlet.2004.04.026. View

Guilhermino L, Lopes M, Carvalho A, Soares A . Inhibition of acetylcholinesterase activity as effect criterion in acute tests with juvenile Daphnia magna. Chemosphere. 1996; 32(4):727-38. DOI: 10.1016/0045-6535(95)00360-6. View

Leticia A, Gerardo G . Determination of esterase activity and characterization of cholinesterases in the reef fish Haemulon plumieri. Ecotoxicol Environ Saf. 2008; 71(3):787-97. DOI: 10.1016/j.ecoenv.2008.01.024. View

Quintaneiro C, Monteiro M, Pastorinho R, Soares A, Nogueira A, Morgado F . Environmental pollution and natural populations: a biomarkers case study from the Iberian Atlantic coast. Mar Pollut Bull. 2006; 52(11):1406-13. DOI: 10.1016/j.marpolbul.2006.04.002. View

Jung J, Addison R, Shim W . Characterization of cholinesterases in marbled sole, Limanda yokohamae, and their inhibition in vitro by the fungicide iprobenfos. Mar Environ Res. 2007; 63(5):471-8. DOI: 10.1016/j.marenvres.2006.12.007. View

Main A, Iverson F . Measurement of the affinity and phosphorylation constants governing irreversible inhibition of cholinesterases by di-isopropyl phosphorofluoridate. Biochem J. 1966; 100(2):525-31. PMC: 1265167. DOI: 10.1042/bj1000525. View

Shaonan L, Xianchuan X, Guonian Z, Yajun T . Kinetic characters and resistance to inhibition of crude and purified brain acetylcholinesterase of three freshwater fishes by organophosphates. Aquat Toxicol. 2004; 68(4):293-9. DOI: 10.1016/j.aquatox.2004.03.013. View

Kitz R, Wilson I . Esters of methanesulfonic acid as irreversible inhibitors of acetylcholinesterase. J Biol Chem. 1962; 237:3245-9. View

Chuiko G, Podgornaya V, Zhelnin Y . Acetylcholinesterase and butyrylcholinesterase activities in brain and plasma of freshwater teleosts: cross-species and cross-family differences. Comp Biochem Physiol B Biochem Mol Biol. 2003; 135(1):55-61. DOI: 10.1016/s1096-4959(03)00048-4. View

10.

Rendon-von Osten J, Ortiz-Arana A, Guilhermino L, Soares A . In vivo evaluation of three biomarkers in the mosquitofish (Gambusia yucatana) exposed to pesticides. Chemosphere. 2004; 58(5):627-36. DOI: 10.1016/j.chemosphere.2004.08.065. View

11.

Johnson J, Wallace K . Species-related differences in the inhibition of brain acetylcholinesterase by paraoxon and malaoxon. Toxicol Appl Pharmacol. 1987; 88(2):234-41. DOI: 10.1016/0041-008x(87)90009-3. View

12.

Arufe M, Arellano J, Garcia L, Albendin G, Sarasquete C . Cholinesterase activity in gilthead seabream (Sparus aurata) larvae: Characterization and sensitivity to the organophosphate azinphosmethyl. Aquat Toxicol. 2007; 84(3):328-336. DOI: 10.1016/j.aquatox.2007.06.009. View

13.

Wang C, Murphy S . Kinetic analysis of species difference in acetylcholinesterase sensitivity to organophosphate insecticides. Toxicol Appl Pharmacol. 1982; 66(3):409-19. DOI: 10.1016/0041-008x(82)90307-6. View

14.

Salles J, Cunha Bastos V, Silva Filho M, Machado O, Salles C, Giovanni de Simone S . A novel butyrylcholinesterase from serum of Leporinus macrocephalus, a Neotropical fish. Biochimie. 2005; 88(1):59-68. DOI: 10.1016/j.biochi.2005.06.017. View

15.

Rodriguez-Fuentes G, Gold-Bouchot G . Characterization of cholinesterase activity from different tissues of Nile tilapia (Oreochromis niloticus). Mar Environ Res. 2004; 58(2-5):505-9. DOI: 10.1016/j.marenvres.2004.03.037. View

16.

de la Torre F, Ferrari L, Salibian A . Freshwater pollution biomarker: response of brain acetylcholinesterase activity in two fish species. Comp Biochem Physiol C Toxicol Pharmacol. 2002; 131(3):271-80. DOI: 10.1016/s1532-0456(02)00014-5. View

17.

Oliveira M, Silva Filho M, Cunha Bastos V, Fernandes F, Bastos J . Brain acetylcholinesterase as a marine pesticide biomarker using Brazilian fishes. Mar Environ Res. 2006; 63(4):303-12. DOI: 10.1016/j.marenvres.2006.10.002. View

18.

Chuiko G . Comparative study of acetylcholinesterase and butyrylcholinesterase in brain and serum of several freshwater fish: specific activities and in vitro inhibition by DDVP, an organophosphorus pesticide. Comp Biochem Physiol C Toxicol Pharmacol. 2001; 127(3):233-42. DOI: 10.1016/s0742-8413(00)00150-x. View

19.

Sole M, Vega S, Varo I . Characterization of type "B" esterases and hepatic CYP450 isoenzimes in Senegalese sole for their further application in monitoring studies. Ecotoxicol Environ Saf. 2011; 78:72-9. DOI: 10.1016/j.ecoenv.2011.11.013. View

20.

Tortelli V, Colares E, Robaldo R, Nery L, Pinho G, Bianchini A . Importance of cholinesterase kinetic parameters in environmental monitoring using estuarine fish. Chemosphere. 2006; 65(4):560-6. DOI: 10.1016/j.chemosphere.2006.02.047. View