Multiphoton Excited Hemoglobin Fluorescence and Third Harmonic Generation for Non-invasive Microscopy of Stored Blood

Overview

Journal Biomed Opt Express

Specialty Radiology

Date 2016 Oct 5

PMID 27699111

Citations 16

Authors

Ilyas Saytashev

Rachel Glenn

Gabrielle A Murashova

Sam Osseiran

Dana Spence

Conor L Evans

Marcos Dantus

Affiliations

Soon will be listed here.

Abstract

Red blood cells (RBC) in two-photon excited fluorescence (TPEF) microscopy usually appear as dark disks because of their low fluorescent signal. Here we use 15fs 800nm pulses for TPEF, 45fs 1060nm pulses for three-photon excited fluorescence, and third harmonic generation (THG) imaging. We find sufficient fluorescent signal that we attribute to hemoglobin fluorescence after comparing time and wavelength resolved spectra of other expected RBC endogenous fluorophores: NADH, FAD, biliverdin, and bilirubin. We find that both TPEF and THG microscopy can be used to examine erythrocyte morphology non-invasively without breaching a blood storage bag.

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