Kidney Tissue Targeted Metabolic Profiling of Unilateral Ureteral Obstruction Rats by NMR

Overview

Journal Front Pharmacol

Date 2016 Oct 4

PMID 27695416

Citations 11

Authors

Zhenyu Li

Aiping Li

Jining Gao

Hong Li

Xuemei Qin

Affiliations

Soon will be listed here.

Abstract

Renal interstitial fibrosis is a common pathological process in the progression of kidney disease. A nuclear magnetic resonance (NMR) based metabolomic approach was used to analyze the kidney tissues of rats with renal interstitial fibrosis (RIF), induced by unilateral ureteral obstruction (UUO). The combination of a variety of statistical methods were used to screen out 14 significantly changed potential metabolites, which are related with multiple biochemical processes including amino acid metabolism, adenine metabolism, energy metabolism, osmolyte change and induced oxidative stress. The exploration of the contralateral kidneys enhanced the understanding of the disease, which was also supported by serum biochemistry and kidney histopathology results. In addition, the pathological parameters (clinical chemistry, histological and immunohistochemistry results) were correlated with the significantly changed differential metabolites related with RIF. This study showed that targeted tissue metabolomic analysis can be used as a useful tool to understand the mechanism of the disease and provide a novel insight in the pathogenesis of RIF.

Citing Articles

Potential candidates from a functional food (Sichuan pepper) for the management of hyperuricemia: high-through virtual screening, network pharmacology and dynamics simulations.

Chen M, Chen X, Chen Q, Chu C, Yang S, Wu C Front Endocrinol (Lausanne). 2024; 15:1436360.

PMID: 39722812 PMC: 11668583. DOI: 10.3389/fendo.2024.1436360.

Molecular mechanisms underlying the role of hypoxia-inducible factor-1 α in metabolic reprogramming in renal fibrosis.

Wei X, Hou Y, Long M, Jiang L, Du Y Front Endocrinol (Lausanne). 2022; 13:927329.

PMID: 35957825 PMC: 9357883. DOI: 10.3389/fendo.2022.927329.

Tissue metabolomics study to reveal the toxicity of a traditional Tibetan medicine 'Renqing Changjue' in rats.

Xu C, Wang Y, Rezeng C, Zhang L, Zhao B, Wang X RSC Adv. 2022; 8(66):37652-37664.

PMID: 35558588 PMC: 9089440. DOI: 10.1039/c8ra07058j.

Sirtuin 3 regulates mitochondrial protein acetylation and metabolism in tubular epithelial cells during renal fibrosis.

Zhang Y, Wen P, Luo J, Ding H, Cao H, He W Cell Death Dis. 2021; 12(9):847.

PMID: 34518519 PMC: 8437958. DOI: 10.1038/s41419-021-04134-4.

Axl-inhibitor bemcentinib alleviates mitochondrial dysfunction in the unilateral ureter obstruction murine model.

Hoel A, Osman T, Hoel F, Elsaid H, Chen T, Landolt L J Cell Mol Med. 2021; 25(15):7407-7417.

PMID: 34219376 PMC: 8335678. DOI: 10.1111/jcmm.16769.

References

Hauet T, Baumert H, Gibelin H, Godart C, Carretier M, Eugene M . Citrate, acetate and renal medullary osmolyte excretion in urine as predictor of renal changes after cold ischaemia and transplantation. Clin Chem Lab Med. 2001; 38(11):1093-8. DOI: 10.1515/CCLM.2000.162. View

Mizuno S, Matsumoto K, Nakamura T . Hepatocyte growth factor suppresses interstitial fibrosis in a mouse model of obstructive nephropathy. Kidney Int. 2001; 59(4):1304-14. DOI: 10.1046/j.1523-1755.2001.0590041304.x. View

Bernal W, Donaldson N, Wyncoll D, Wendon J . Blood lactate as an early predictor of outcome in paracetamol-induced acute liver failure: a cohort study. Lancet. 2002; 359(9306):558-63. DOI: 10.1016/S0140-6736(02)07743-7. View

Asensi M, Marquez R, Martinez-Valls J, Vento M, Pallardo F, Sastre J . Xanthine oxidase is involved in free radical production in type 1 diabetes: protection by allopurinol. Diabetes. 2002; 51(4):1118-24. DOI: 10.2337/diabetes.51.4.1118. View

Taes Y, Delanghe J, De Vriese A, Rombaut R, Van Camp J, Lameire N . Creatine supplementation decreases homocysteine in an animal model of uremia. Kidney Int. 2003; 64(4):1331-7. DOI: 10.1046/j.1523-1755.2003.00206.x. View

Liu Y . Hepatocyte growth factor in kidney fibrosis: therapeutic potential and mechanisms of action. Am J Physiol Renal Physiol. 2004; 287(1):F7-16. DOI: 10.1152/ajprenal.00451.2003. View

Serkova N, Fuller T, Klawitter J, Freise C, Niemann C . H-NMR-based metabolic signatures of mild and severe ischemia/reperfusion injury in rat kidney transplants. Kidney Int. 2005; 67(3):1142-51. DOI: 10.1111/j.1523-1755.2005.00181.x. View

Waters N, Waterfield C, Farrant R, Holmes E, Nicholson J . Metabonomic deconvolution of embedded toxicity: application to thioacetamide hepato- and nephrotoxicity. Chem Res Toxicol. 2005; 18(4):639-54. DOI: 10.1021/tx049869b. View

Liu Y, Yang J . Hepatocyte growth factor: new arsenal in the fights against renal fibrosis?. Kidney Int. 2006; 70(2):238-40. DOI: 10.1038/sj.ki.5001661. View

10.

Liao P, Wei L, Zhang X, Li X, Wu H, Wu Y . Metabolic profiling of serum from gadolinium chloride-treated rats by 1H NMR spectroscopy. Anal Biochem. 2007; 364(2):112-21. DOI: 10.1016/j.ab.2007.02.020. View

11.

Fredholm B . Adenosine, an endogenous distress signal, modulates tissue damage and repair. Cell Death Differ. 2007; 14(7):1315-23. DOI: 10.1038/sj.cdd.4402132. View

12.

Meng L, Qu L, Tang J, Cai S, Wang H, Li X . A combination of Chinese herbs, Astragalus membranaceus var. mongholicus and Angelica sinensis, enhanced nitric oxide production in obstructed rat kidney. Vascul Pharmacol. 2007; 47(2-3):174-83. DOI: 10.1016/j.vph.2007.06.002. View

13.

Niemann C, Serkova N . Biochemical mechanisms of nephrotoxicity: application for metabolomics. Expert Opin Drug Metab Toxicol. 2007; 3(4):527-44. DOI: 10.1517/17425225.3.4.527. View

14.

Ma H, Sorokin A, Mazein A, Selkov A, Selkov E, Demin O . The Edinburgh human metabolic network reconstruction and its functional analysis. Mol Syst Biol. 2007; 3:135. PMC: 2013923. DOI: 10.1038/msb4100177. View

15.

Markley J, Anderson M, Cui Q, Eghbalnia H, Lewis I, Hegeman A . New bioinformatics resources for metabolomics. Pac Symp Biocomput. 2007; :157-68. View

16.

Kandar R, Zakova P . Allantoin as a marker of oxidative stress in human erythrocytes. Clin Chem Lab Med. 2008; 46(9):1270-4. DOI: 10.1515/CCLM.2008.244. View

17.

Lei R, Wu C, Yang B, Ma H, Shi C, Wang Q . Integrated metabolomic analysis of the nano-sized copper particle-induced hepatotoxicity and nephrotoxicity in rats: a rapid in vivo screening method for nanotoxicity. Toxicol Appl Pharmacol. 2008; 232(2):292-301. DOI: 10.1016/j.taap.2008.06.026. View

18.

Wishart D, Knox C, Guo A, Eisner R, Young N, Gautam B . HMDB: a knowledgebase for the human metabolome. Nucleic Acids Res. 2008; 37(Database issue):D603-10. PMC: 2686599. DOI: 10.1093/nar/gkn810. View

19.

Xie X, Yang M, Liu H, Zuo C, Li Z, Deng Y . Influence of ginsenoside Rg1, a panaxatriol saponin from Panax notoginseng, on renal fibrosis in rats with unilateral ureteral obstruction. J Zhejiang Univ Sci B. 2008; 9(11):885-94. PMC: 2579952. DOI: 10.1631/jzus.B0820024. View

20.

Hewitson T . Renal tubulointerstitial fibrosis: common but never simple. Am J Physiol Renal Physiol. 2009; 296(6):F1239-44. DOI: 10.1152/ajprenal.90521.2008. View