Effects of Citric and Lactic Acid on the Reduction of Deoxynivalenol and Its Derivatives in Feeds

Overview

Journal Toxins (Basel)

Publisher MDPI

Specialty Toxicology

Date 2016 Oct 1

PMID 27690101

Citations 10

Authors

Elke Humer

Annegret Lucke

Hauke Harder

Barbara U Metzler-Zebeli

Josef Bohm

Qendrim Zebeli

Affiliations

Soon will be listed here.

Abstract

Exposure to mycotoxin-contaminated feeds represents a serious health risk. This has necessitated the need for the establishment of practical methods for mycotoxin decontamination. This study investigated the effects of citric acid (CA) and lactic acid (LA) on common trichothecene mycotoxins in feeds contaminated with mycotoxins. Contaminated feed samples were processed either with 5% CA or 5% LA solutions in a ratio of 1:1.2 (/) for 5, 24, or 48 h, and analyzed for multiple mycotoxin metabolites using a liquid chromatography-tandem mass spectrometric method. The analyses showed that treating the feed with CA and LA lowered the concentration of deoxynivalenol (DON), whereby 5% LA lowered the original DON concentration in the contaminated feed samples by half, irrespective of the processing time. Similar lowering effects were observed for the concentrations of 15Ac-DON, 5-hydroxyculmorin, and sambucinol. The concentration of nivalenol was only lowered by the LA treatment. In contrast, CA and LA treatments showed no or only small effects on the concentration of several mycotoxins and their derivatives, including zearalenone, fumonisins, and culmorin. In conclusion, the present results indicate that the use of 5% solutions of LA and CA might reduce the concentration of common trichothecene mycotoxins, especially DON and its derivate 15Ac-DON. However, further research is required to determine the effect on overall toxicity and to identify the underlying mechanisms.

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References

Plasencia J, Mirocha C . Isolation and characterization of zearalenone sulfate produced by Fusarium spp. Appl Environ Microbiol. 1991; 57(1):146-50. PMC: 182675. DOI: 10.1128/aem.57.1.146-150.1991. View

Streit E, Schwab C, Sulyok M, Naehrer K, Krska R, Schatzmayr G . Multi-mycotoxin screening reveals the occurrence of 139 different secondary metabolites in feed and feed ingredients. Toxins (Basel). 2013; 5(3):504-23. PMC: 3705275. DOI: 10.3390/toxins5030504. View

Ghareeb K, Awad W, Bohm J, Zebeli Q . Impacts of the feed contaminant deoxynivalenol on the intestine of monogastric animals: poultry and swine. J Appl Toxicol. 2014; 35(4):327-37. DOI: 10.1002/jat.3083. View

Jalili M, Jinap S, Son R . The effect of chemical treatment on reduction of aflatoxins and ochratoxin A in black and white pepper during washing. Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2011; 28(4):485-93. DOI: 10.1080/19440049.2010.551300. View

Zamir L, Nikolakakis A, Huang L, St-Pierre P, Sauriol F, Sparace S . Biosynthesis of 3-acetyldeoxynivalenol and sambucinol. Identification of the two oxygenation steps after trichodiene. J Biol Chem. 1999; 274(18):12269-77. DOI: 10.1074/jbc.274.18.12269. View

Sulyok M, Berthiller F, Krska R, Schuhmacher R . Development and validation of a liquid chromatography/tandem mass spectrometric method for the determination of 39 mycotoxins in wheat and maize. Rapid Commun Mass Spectrom. 2006; 20(18):2649-59. DOI: 10.1002/rcm.2640. View

Nelson P, Dignani M, Anaissie E . Taxonomy, biology, and clinical aspects of Fusarium species. Clin Microbiol Rev. 1994; 7(4):479-504. PMC: 358338. DOI: 10.1128/CMR.7.4.479. View

Escriva L, Font G, Manyes L . In vivo toxicity studies of fusarium mycotoxins in the last decade: a review. Food Chem Toxicol. 2015; 78:185-206. DOI: 10.1016/j.fct.2015.02.005. View

Sundstol Eriksen G, Pettersson H, Lundh T . Comparative cytotoxicity of deoxynivalenol, nivalenol, their acetylated derivatives and de-epoxy metabolites. Food Chem Toxicol. 2004; 42(4):619-24. DOI: 10.1016/j.fct.2003.11.006. View

10.

Mendez-Albores A, Arambula-Villa G, Loarca-Pina M, Castano-Tostado E, Moreno-Martinez E . Safety and efficacy evaluation of aqueous citric acid to degrade B-aflatoxins in maize. Food Chem Toxicol. 2004; 43(2):233-8. DOI: 10.1016/j.fct.2004.09.009. View

11.

Diaz G, Krska R, Sulyok M . Mycotoxins and cyanogenic glycosides in staple foods of three indigenous people of the Colombian Amazon. Food Addit Contam Part B Surveill. 2015; 8(4):291-7. DOI: 10.1080/19393210.2015.1089948. View

12.

Marin S, Ramos A, Cano-Sancho G, Sanchis V . Mycotoxins: occurrence, toxicology, and exposure assessment. Food Chem Toxicol. 2013; 60:218-37. DOI: 10.1016/j.fct.2013.07.047. View

13.

van der Merwe K, Steyn P, Fourie L . Mycotoxins. II. The constitution of ochratoxins A, B, and C, metabolites of Aspergillus ochraceus Wilh. J Chem Soc Perkin 1. 1965; :7083-8. DOI: 10.1039/jr9650007083. View

14.

Juan C, Ritieni A, Manes J . Determination of trichothecenes and zearalenones in grain cereal, flour and bread by liquid chromatography tandem mass spectrometry. Food Chem. 2013; 134(4):2389-97. DOI: 10.1016/j.foodchem.2012.04.051. View

15.

Seeboth J, Solinhac R, Oswald I, Guzylack-Piriou L . The fungal T-2 toxin alters the activation of primary macrophages induced by TLR-agonists resulting in a decrease of the inflammatory response in the pig. Vet Res. 2012; 43:35. PMC: 3416672. DOI: 10.1186/1297-9716-43-35. View

16.

Ghebremeskel M, Langseth W . The occurrence of culmorin and hydroxy-culmorins in cereals. Mycopathologia. 2002; 152(2):103-8. DOI: 10.1023/a:1012479823193. View

17.

Berthiller F, Krska R, Domig K, Kneifel W, Juge N, Schuhmacher R . Hydrolytic fate of deoxynivalenol-3-glucoside during digestion. Toxicol Lett. 2011; 206(3):264-7. PMC: 3185207. DOI: 10.1016/j.toxlet.2011.08.006. View

18.

Kabak B, Dobson A, Var I . Strategies to prevent mycotoxin contamination of food and animal feed: a review. Crit Rev Food Sci Nutr. 2006; 46(8):593-619. DOI: 10.1080/10408390500436185. View

19.

Hellwig V, Grothe T, Mayer-Bartschmid A, Endermann R, Henkel T, Stadler M . Altersetin, a new antibiotic from cultures of endophytic Alternaria spp. Taxonomy, fermentation, isolation, structure elucidation and biological activities. J Antibiot (Tokyo). 2003; 55(10):881-92. DOI: 10.7164/antibiotics.55.881. View

20.

Mendez-Albores A, Martinez-Bustos F, Gaytan-Martinez M, Moreno-Martinez E . Effect of lactic and citric acid on the stability of B-aflatoxins in extrusion-cooked sorghum. Lett Appl Microbiol. 2008; 47(1):1-7. DOI: 10.1111/j.1472-765X.2008.02376.x. View