Sonoreperfusion Therapy Kinetics in Whole Blood Using Ultrasound, Microbubbles and Tissue Plasminogen Activator

Overview

Journal Ultrasound Med Biol

Specialty Radiology

Date 2016 Oct 1

PMID 27687734

Citations 7

Authors

Sebastiaan T Roos

Francois T Yu

Otto Kamp

Xucai Chen

Flordeliza S Villanueva

John J Pacella

Affiliations

Soon will be listed here.

Abstract

Coronary intervention for myocardial infarction often results in microvascular embolization of thrombus. Sonoreperfusion therapy (SRP) using ultrasound and microbubbles restored perfusion in our in vitro flow model of microvascular obstruction. In this study, we assessed SRP efficacy using whole blood as the perfusate with and without tissue plasminogen activator (tPA). In a phantom vessel bearing a 40-μm-pore mesh to simulate the microvasculature, microthrombi were injected to cause microvascular obstruction and were treated using SRP. Without tPA, the lytic rate increased from 2.6 ± 1.5 mmHg/min with 1000-cycle pulses to 7.3 ± 3.2 mmHg/min with 5000-cycle ultrasound pulses (p < 0.01). The lytic index was similar for tPA-only ([2.0 ± 0.5] × 10 mmHg min) and 5000 cycles without tPA ([2.3 ± 0.5] × 10 mmHg min) (p = 0.5) but increased ([3.6 ± 0.8] × 10 mmHg min) with tPA in conjunction with 5000-cycles ultrasound (p < 0.01). In conclusion, SRP restored microvascular perfusion in whole blood, SRP lytic rate in experiments without tPA increased with ultrasound pulse length and efficacy increased with the addition of tPA.

Citing Articles

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