POTENTIAL CROSS-CONTAMINATION OF SIMILAR Giardia Duodenalis ASSEMBLAGE IN CHILDREN AND PET DOGS IN SOUTHERN BRAZIL, AS DETERMINED BY PCR-RFLP

Overview

Journal Rev Inst Med Trop Sao Paulo

Specialty Tropical Medicine

Date 2016 Sep 30

PMID 27680171

Citations 10

Authors

Rosileia Marinho de Quadros

Paulo Henrique Exterchoter Weiss

Sandra Marcia Tietz Marques

Luiz Claudio Miletti

Affiliations

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Abstract

Giardia duodenalis is an enteric parasite that has distinct genetic groups. Human infections are mainly caused by assemblages A and B, although sporadic infections by assemblages C and D have also been reported. Animals can be infected by a wide range of assemblages (A to H). The aim of this study is to identify the assemblages and sub-assemblages of G. duodenalis with zoonotic features in fecal samples of school-aged children, and in dogs that coexist in the same households in Lages, Santa Catarina, Brazil. Fecal samples of 91 children and 108 dogs were obtained and G. duodenalis cysts were detected in samples from 11 (12.08%) children and 10 (9.25%) dogs. DNA extracted from the 21 positive samples was analyzed by PCR-RFLP, using the gdh gene. Results showed the presence of sub-assemblages AI (2/11), AII (4/11), BIII (2/11), and BIV(3/11) among children and AI (5/10) and BIV(3/10) in dogs, with zoonotic characteristics, and the carnivore specific assemblage C (2/10). G. duodenalis was found to infect both children and dogs living in the same household, with the same sub-assemblage (BIV) indicating that pet dogs are a potential risk of transmission of G. duodenalis to humans.

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References

Lasek-Nesselquist E, Welch D, Sogin M . The identification of a new Giardia duodenalis assemblage in marine vertebrates and a preliminary analysis of G. duodenalis population biology in marine systems. Int J Parasitol. 2010; 40(9):1063-74. PMC: 2900473. DOI: 10.1016/j.ijpara.2010.02.015. View

Prado M, Strina A, Barreto M, Oliveira-Assis A, Paz L, Cairncross S . Risk factors for infection with Giardia duodenalis in pre-school children in the city of Salvador, Brazil. Epidemiol Infect. 2003; 131(2):899-906. PMC: 2870034. DOI: 10.1017/s0950268803001018. View

Sahagun J, Clavel A, Goni P, Seral C, Llorente M, Castillo F . Correlation between the presence of symptoms and the Giardia duodenalis genotype. Eur J Clin Microbiol Infect Dis. 2007; 27(1):81-3. DOI: 10.1007/s10096-007-0404-3. View

Souza S, Gennari S, Richtzenhain L, Pena H, Funada M, Cortez A . Molecular identification of Giardia duodenalis isolates from humans, dogs, cats and cattle from the state of São Paulo, Brazil, by sequence analysis of fragments of glutamate dehydrogenase (gdh) coding gene. Vet Parasitol. 2007; 149(3-4):258-64. DOI: 10.1016/j.vetpar.2007.08.019. View

Mahdy A, Surin J, Wan K, Mohd-Adnan A, Al-Mekhlafi M, Lim Y . Giardia intestinalis genotypes: Risk factors and correlation with clinical symptoms. Acta Trop. 2009; 112(1):67-70. DOI: 10.1016/j.actatropica.2009.06.012. View

Kohli A, Bushen O, Pinkerton R, Houpt E, Newman R, Sears C . Giardia duodenalis assemblage, clinical presentation and markers of intestinal inflammation in Brazilian children. Trans R Soc Trop Med Hyg. 2008; 102(7):718-25. PMC: 2963065. DOI: 10.1016/j.trstmh.2008.03.002. View

Katagiri S, Oliveira-Sequeira T . Prevalence of dog intestinal parasites and risk perception of zoonotic infection by dog owners in São Paulo State, Brazil. Zoonoses Public Health. 2008; 55(8-10):406-13. DOI: 10.1111/j.1863-2378.2008.01163.x. View

Ey P, Mansouri M, Kulda J, Nohynkova E, Monis P, Andrews R . Genetic analysis of Giardia from hoofed farm animals reveals artiodactyl-specific and potentially zoonotic genotypes. J Eukaryot Microbiol. 1998; 44(6):626-35. DOI: 10.1111/j.1550-7408.1997.tb05970.x. View

Traub R, Inpankaew T, Reid S, Sutthikornchai C, Sukthana Y, Robertson I . Transmission cycles of Giardia duodenalis in dogs and humans in Temple communities in Bangkok--a critical evaluation of its prevalence using three diagnostic tests in the field in the absence of a gold standard. Acta Trop. 2009; 111(2):125-32. DOI: 10.1016/j.actatropica.2009.03.006. View

10.

Eligio-Garcia L, Cortes-Campos A, Jimenez-Cardoso E . Classification of Giardia intestinalis isolates by multiple polymerase chain reaction (multiplex). Parasitol Res. 2008; 103(4):797-800. DOI: 10.1007/s00436-008-1042-0. View

11.

Kirkpatrick C . Giardiasis. Vet Clin North Am Small Anim Pract. 1987; 17(6):1377-87. DOI: 10.1016/s0195-5616(87)50007-9. View

12.

Thompson R, Smith A, Lymbery A, Averis S, Morris K, Wayne A . Giardia in Western Australian wildlife. Vet Parasitol. 2010; 170(3-4):207-11. DOI: 10.1016/j.vetpar.2010.02.012. View

13.

Savioli L, Smith H, Thompson A . Giardia and Cryptosporidium join the 'Neglected Diseases Initiative'. Trends Parasitol. 2006; 22(5):203-8. DOI: 10.1016/j.pt.2006.02.015. View

14.

Volotao A, Costa-Macedo L, Haddad F, Brandao A, Peralta J, Fernandes O . Genotyping of Giardia duodenalis from human and animal samples from Brazil using beta-giardin gene: a phylogenetic analysis. Acta Trop. 2007; 102(1):10-9. DOI: 10.1016/j.actatropica.2007.02.010. View

15.

Amar C, Dear P, McLauchlin J . Detection and genotyping by real-time PCR/RFLP analyses of Giardia duodenalis from human faeces. J Med Microbiol. 2003; 52(Pt 8):681-683. DOI: 10.1099/jmm.0.05193-0. View

16.

Traub R, Monis P, Robertson I, Irwin P, Mencke N, Thompson R . Epidemiological and molecular evidence supports the zoonotic transmission of Giardia among humans and dogs living in the same community. Parasitology. 2004; 128(Pt 3):253-62. DOI: 10.1017/s0031182003004505. View

17.

Khan S, Debnath C, Pramanik A, Xiao L, Nozaki T, Ganguly S . Molecular evidence for zoonotic transmission of Giardia duodenalis among dairy farm workers in West Bengal, India. Vet Parasitol. 2011; 178(3-4):342-5. DOI: 10.1016/j.vetpar.2011.01.029. View

18.

Aydin A, Besirbellioglu B, Avci I, Tanyuksel M, Araz E, Pahsa A . Classification of Giardia duodenalis parasites in Turkey into groups A and B using restriction fragment length polymorphism. Diagn Microbiol Infect Dis. 2004; 50(2):147-51. DOI: 10.1016/j.diagmicrobio.2004.06.001. View

19.

Inpankaew T, Traub R, Thompson R, Sukthana Y . Canine parasitic zoonoses in Bangkok temples. Southeast Asian J Trop Med Public Health. 2007; 38(2):247-55. View

20.

Homan W, Mank T . Human giardiasis: genotype linked differences in clinical symptomatology. Int J Parasitol. 2001; 31(8):822-6. DOI: 10.1016/s0020-7519(01)00183-7. View