Protective Effects of Gallic Acid Against NiSO4-Induced Toxicity Through Down-Regulation of the Ras/ERK Signaling Pathway in Beas-2B Cells

Overview

Journal Med Sci Monit

Specialties General Medicine
Pathology

Date 2016 Sep 28

PMID 27676106

Citations 2

Authors

Xuejun An

Aijia Zhou

Yue Yang

Yue Wang

Rui Xin

Chao Tian

Yonghui Wu

Affiliations

Soon will be listed here.

Abstract

BACKGROUND This study aimed to explore the preventive effects of gallic acid (GA) on the toxicity induced by NiSO4 in Beas-2B cells. MATERIAL AND METHODS Beas-2B cell viability was measured by MTT assay. The degree of oxidative stress was detected by measuring the levels of reactive oxygen species (ROS) and lipid peroxide (LPO). The rate of apoptosis was measured by flow cytometry. Ras/ERK-related protein levels were analyzed by Western blot analysis, which including Ras, ERK, c-Myc, PARP, and PARP cleavage. RESULTS MTT assay showed that NiSO4 induced cytotoxicity, while GA had a protective role against toxicity. Additionally, GA could reduce the apoptotic cell number and the level of ROS in Beas-2B cells induced by NiSO4. Western blot analysis demonstrated that NiSO4 could up-regulate the related protein in the Ras/ERK signaling pathway. Furthermore, we observed that GA could alleviate the toxicity of NiSO4 through regulating protein changes in the Ras/ERK signaling pathway. CONCLUSIONS Preventive effects of GA on NiSO4-induced cytotoxicity in Beas-2B cells may be through the Ras/ERK signaling pathways.

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References

Ahamed M, Akhtar M, Siddiqui M, Ahmad J, Musarrat J, Al-Khedhairy A . Oxidative stress mediated apoptosis induced by nickel ferrite nanoparticles in cultured A549 cells. Toxicology. 2011; 283(2-3):101-8. DOI: 10.1016/j.tox.2011.02.010. View

Manach C, Williamson G, Morand C, Scalbert A, Remesy C . Bioavailability and bioefficacy of polyphenols in humans. I. Review of 97 bioavailability studies. Am J Clin Nutr. 2005; 81(1 Suppl):230S-242S. DOI: 10.1093/ajcn/81.1.230S. View

Ma L, Bai Y, Pu H, He J, Bassig B, Dai M . A retrospective cohort mortality study in Jinchang, the largest nickel production enterprise in China. Biomed Environ Sci. 2014; 27(7):567-71. DOI: 10.3967/bes2014.088. View

Luscher B, Larsson L . The world according to MYC. Conference on MYC and the transcriptional control of proliferation and oncogenesis. EMBO Rep. 2007; 8(12):1110-4. PMC: 2267245. DOI: 10.1038/sj.embor.7401121. View

Guan F, Zhang D, Wang X, Chen J . Nitric oxide and bcl-2 mediated the apoptosis induced by nickel(II) in human T hybridoma cells. Toxicol Appl Pharmacol. 2007; 221(1):86-94. DOI: 10.1016/j.taap.2007.01.029. View

Ke Q, Li Q, Ellen T, Sun H, Costa M . Nickel compounds induce phosphorylation of histone H3 at serine 10 by activating JNK-MAPK pathway. Carcinogenesis. 2008; 29(6):1276-81. PMC: 2829883. DOI: 10.1093/carcin/bgn084. View

Lu S, Zhang W, Zhang R, Liu P, Wang Q, Shang Y . Comparison of cellular toxicity caused by ambient ultrafine particles and engineered metal oxide nanoparticles. Part Fibre Toxicol. 2015; 12:5. PMC: 4412114. DOI: 10.1186/s12989-015-0082-8. View

Wu B, Cui H, Peng X, Fang J, Zuo Z, Deng J . Dietary nickel chloride induces oxidative stress, apoptosis and alters Bax/Bcl-2 and caspase-3 mRNA expression in the cecal tonsil of broilers. Food Chem Toxicol. 2013; 63:18-29. DOI: 10.1016/j.fct.2013.10.033. View

Doreswamy K, Shrilatha B, Rajeshkumar T, Muralidhara . Nickel-induced oxidative stress in testis of mice: evidence of DNA damage and genotoxic effects. J Androl. 2004; 25(6):996-1003. DOI: 10.1002/j.1939-4640.2004.tb03173.x. View

10.

DOLL R, Morgan L, Speizer F . Cancers of the lung and nasal sinuses in nickel workers. Br J Cancer. 1970; 24(4):623-32. PMC: 2008725. DOI: 10.1038/bjc.1970.76. View

11.

Freitas M, Barcellos-de-Souza P, Barja-Fidalgo C, Fernandes E . Nickel induces apoptosis in human neutrophils. Biometals. 2012; 26(1):13-21. DOI: 10.1007/s10534-012-9590-2. View

12.

Simko M, Mattsson M . Extremely low frequency electromagnetic fields as effectors of cellular responses in vitro: possible immune cell activation. J Cell Biochem. 2004; 93(1):83-92. DOI: 10.1002/jcb.20198. View

13.

Murakawa M, Jung S, Iijima K, Yonehara S . Apoptosis-inducing protein, AIP, from parasite-infected fish induces apoptosis in mammalian cells by two different molecular mechanisms. Cell Death Differ. 2001; 8(3):298-307. DOI: 10.1038/sj.cdd.4400811. View

14.

Valeeva E, Galimova R, Karimova L, Muldashova N . [Cases of nickel carbonyl acute poisoning at major petrochemical enterprises]. Med Tr Prom Ekol. 2010; (11):17-9. View

15.

Huang J, Cui H, Peng X, Fang J, Zuo Z, Deng J . The association between splenocyte apoptosis and alterations of Bax, Bcl-2 and caspase-3 mRNA expression, and oxidative stress induced by dietary nickel chloride in broilers. Int J Environ Res Public Health. 2013; 10(12):7310-26. PMC: 3881169. DOI: 10.3390/ijerph10127310. View

16.

Chakrabarti S, Bai C, Subramanian K . DNA-protein crosslinks induced by nickel compounds in isolated rat lymphocytes: role of reactive oxygen species and specific amino acids. Toxicol Appl Pharmacol. 2001; 170(3):153-65. DOI: 10.1006/taap.2000.9097. View

17.

Grandjean P . Human exposure to nickel. IARC Sci Publ. 1984; (53):469-85. View

18.

Ohno T, Inoue M, Ogihara Y . Cytotoxic activity of gallic acid against liver metastasis of mastocytoma cells P-815. Anticancer Res. 2002; 21(6A):3875-80. View

19.

Li Q, Suen T, Sun H, Arita A, Costa M . Nickel compounds induce apoptosis in human bronchial epithelial Beas-2B cells by activation of c-Myc through ERK pathway. Toxicol Appl Pharmacol. 2009; 235(2):191-8. PMC: 2657554. DOI: 10.1016/j.taap.2008.12.005. View

20.

Cavallo D, Ursini C, Setini A, Chianese C, Piegari P, Perniconi B . Evaluation of oxidative damage and inhibition of DNA repair in an in vitro study of nickel exposure. Toxicol In Vitro. 2003; 17(5-6):603-7. DOI: 10.1016/s0887-2333(03)00138-3. View