Three-dimensional MicroCT Imaging of Mouse Development from Early Post-implantation to Early Postnatal Stages

Overview

Journal Dev Biol

Publisher Elsevier

Specialties Biology
Reproductive Medicine

Date 2016 Sep 28

PMID 27671873

Citations 34

Authors

Chih-Wei Hsu

Leeyean Wong

Tara L Rasmussen

Sowmya Kalaga

Melissa L McElwee

Lance C Keith

Ritu Bohat

John R Seavitt

Arthur L Beaudet

Mary E Dickinson

Affiliations

Soon will be listed here.

Abstract

In this work, we report the use of iodine-contrast microCT to perform high-throughput 3D morphological analysis of mouse embryos and neonates between embryonic day 8.5 to postnatal day 3, with high spatial resolution up to 3µm/voxel. We show that mouse embryos at early stages can be imaged either within extra embryonic tissues such as the yolk sac or the decidua without physically disturbing the embryos. This method enables a full, undisturbed analysis of embryo turning, allantois development, vitelline vessels remodeling, yolk sac and early placenta development, which provides increased insights into early embryonic lethality in mutant lines. Moreover, these methods are inexpensive, simple to learn and do not require substantial processing time, making them ideal for high throughput analysis of mouse mutants with embryonic and early postnatal lethality.

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