Quantitative Chemical Exchange Saturation Transfer MRI of Intervertebral Disc in a Porcine Model

Overview

Journal Magn Reson Med

Publisher Wiley

Specialty Radiology

Date 2016 Sep 28

PMID 27670140

Citations 12

Authors

Zhengwei Zhou

Maxim Bez

Wafa Tawackoli

Joseph Giaconi

Dmitriy Sheyn

Sandra de Mel

Marcel M Maya

Barry D Pressman

Zulma Gazit

Gadi Pelled

Dan Gazit

Debiao Li

Affiliations

Soon will be listed here.

Abstract

Purpose: Previous studies have associated low pH in intervertebral discs (IVDs) with discogenic back pain. The purpose of this study was to determine whether quantitative CEST (qCEST) MRI can be used to detect pH changes in IVDs in vivo.

Methods: The exchange rate k between glycosaminoglycan (GAG) protons and water protons was determined from qCEST analysis. Its dependence on pH value was investigated in GAG phantoms with varying pH and concentrations. The relationship between k and pH was studied further in vivo in a porcine model on a 3T MR scanner and validated using a pH meter. Sodium lactate was injected into the IVDs to induce various pH values within the discs ranging from 5 to 7.

Results: Phantom and animal results revealed that k measured using qCEST MRI is highly correlated with pH level. In the animal studies, the relationship can be described as k =9.2 × 10 × 10 + 196.9, R = 0.7883.

Conclusion: The exchange rate between GAG and water protons determined from qCEST MRI is closely correlated with pH value. This technique has the potential to noninvasively measure pH in the IVDs of patients with discogenic pain. Magn Reson Med 76:1677-1683, 2016. © 2016 International Society for Magnetic Resonance in Medicine.

Citing Articles

Lorentzian-Corrected Apparent Exchange-Dependent Relaxation (LAREX) Ω-Plot Analysis-An Adaptation for qCEST in a Multi-Pool System: Comprehensive In Silico, In Situ, and In Vivo Studies.

Radke K, Wilms L, Frenken M, Stabinska J, Knet M, Kamp B Int J Mol Sci. 2022; 23(13).

PMID: 35805925 PMC: 9266897. DOI: 10.3390/ijms23136920.

Intradiscal quantitative chemical exchange saturation transfer MRI signal correlates with discogenic pain in human patients.

Pelled G, Salas M, Han P, Gill 3rd H, Lautenschlager K, Lai T Sci Rep. 2021; 11(1):19195.

PMID: 34584114 PMC: 8478892. DOI: 10.1038/s41598-021-97672-y.

A comprehensive tool box for large animal studies of intervertebral disc degeneration.

Lee N, Salzer E, Bach F, Bonilla A, Cook J, Gazit Z JOR Spine. 2021; 4(2):e1162.

PMID: 34337336 PMC: 8313180. DOI: 10.1002/jsp2.1162.

Characterization of microenvironmental changes in the intervertebral discs of patients with chronic low back pain using multiparametric MRI contrasts extracted from Z-spectrum.

Li L, Zhou Z, Xiong W, Fang J, Scotti A, Shaghaghi M Eur Spine J. 2021; 30(4):1063-1071.

PMID: 33475842 PMC: 11421479. DOI: 10.1007/s00586-021-06733-3.

Micro Fragmented Adipose Tissue Promotes the Matrix Synthesis Function of Nucleus Pulposus Cells and Regenerates Degenerated Intervertebral Disc in a Pig Model.

Zhou X, Zhang F, Wang D, Wang J, Wang C, Xia K Cell Transplant. 2020; 29:963689720905798.

PMID: 32030997 PMC: 7444234. DOI: 10.1177/0963689720905798.

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