Differentiation of Normal and Leukemic Cells by 2D Light Scattering Label-free Static Cytometry

Overview

Journal Opt Express

Specialties Biophysics
Ophthalmology

Date 2016 Sep 24

PMID 27661908

Citations 8

Authors

Linyan Xie

Qiao Liu

Changshun Shao

Xuantao Su

Affiliations

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Abstract

Two-dimensional (2D) light scattering patterns of single microspheres, normal granulocytes and leukemic cells are obtained by label-free static cytometry. Statistical results of experimental 2D light scattering patterns obtained from standard microspheres with a mean diameter of 4.19 μm agree well with theoretical simulations. High accuracy rates (greater than 92%) for label-free differentiation of normal granulocytes and leukemic cells, both the acute and chronic leukemic cells, are achieved by analyzing the 2D light scattering patterns. Our label-free static cytometry is promising for leukemia screening in clinics.

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