» Articles » PMID: 27660926

Altered Biomarkers in Trophoblast Cells Obtained Noninvasively Prior to Clinical Manifestation of Perinatal Disease

Overview
Journal Sci Rep
Specialty Science
Date 2016 Sep 24
PMID 27660926
Citations 15
Authors
Affiliations
Soon will be listed here.
Abstract

A contributing factor to poor placental perfusion, leading to intrauterine growth restriction and preeclampsia, is the failure of invading extravillous trophoblast (EVT) cells to remodel the maternal uterine arteries during the first and second trimesters of pregnancy. Noninvasive assessment of EVT cells in ongoing pregnancies is possible beginning three weeks after conception, using trophoblast retrieval and isolation from the cervix (TRIC). Seven proteins were semi-quantified by immunofluorescence microscopy in EVT cells obtained between gestational weeks 6 and 20 from pregnancies with normal outcomes (N = 29) and those with intrauterine growth restriction or preeclampsia (N = 12). Significant differences were measured in expression of PAPPA, FLT1, ENG, AFP, PGF, and LGALS14, but not LGALS13 or the lineage marker KRT7. These findings provide for the first time direct evidence of pathology-associated protein dysregulation in EVT cells during early placentation. The TRIC platform provides a novel approach to acquire molecular signatures of EVT cells that can be correlated with pregnancy outcome.

Citing Articles

Cell-based Noninvasive Prenatal Testing (cbNIPT)-A Review on the Current Developments and Future Prospects.

Maktabi M, Vossaert L, Van den Veyver I Clin Obstet Gynecol. 2023; 66(3):636-648.

PMID: 37650673 PMC: 10491429. DOI: 10.1097/GRF.0000000000000798.


A Novel Paradigm for Non-Invasive Prenatal Genetic Screening: Trophoblast Retrieval and Isolation from the Cervix (TRIC).

Hong K, Park H, Jang H, Shim S, Jang Y, Kim S Diagnostics (Basel). 2023; 13(15).

PMID: 37568895 PMC: 10417081. DOI: 10.3390/diagnostics13152532.


Establishment of the fetal-maternal interface: developmental events in human implantation and placentation.

Huang C, Hsueh Y, Chang C, Hsu H, Yang T, Lin W Front Cell Dev Biol. 2023; 11:1200330.

PMID: 37266451 PMC: 10230101. DOI: 10.3389/fcell.2023.1200330.


Placental protein levels in maternal serum are associated with adverse pregnancy outcomes in nulliparous patients.

Parry S, Carper B, Grobman W, Wapner R, Chung J, Haas D Am J Obstet Gynecol. 2022; 227(3):497.e1-497.e13.

PMID: 35487327 PMC: 9420814. DOI: 10.1016/j.ajog.2022.03.064.


Overview and recent developments in cell-based noninvasive prenatal testing.

Vossaert L, Chakchouk I, Zemet R, Van den Veyver I Prenat Diagn. 2021; 41(10):1202-1214.

PMID: 33974713 PMC: 9355411. DOI: 10.1002/pd.5957.


References
1.
Than N, Romero R, Xu Y, Erez O, Xu Z, Bhatti G . Evolutionary origins of the placental expression of chromosome 19 cluster galectins and their complex dysregulation in preeclampsia. Placenta. 2014; 35(11):855-65. PMC: 4203431. DOI: 10.1016/j.placenta.2014.07.015. View

2.
Gao J, Shen J, Jiang Y, Zhou X, Qi H, Liu X . [Value of second trimester maternal serum sFlt-1, PlGF and their ratio in the prediction of preeclampsia]. Zhonghua Fu Chan Ke Za Zhi. 2014; 49(1):22-5. View

3.
Fejgin M, Diukman R, Cotton Y, Weinstein G, Amiel A . Fetal cells in the uterine cervix: a source for early non-invasive prenatal diagnosis. Prenat Diagn. 2001; 21(8):619-21. DOI: 10.1002/pd.117. View

4.
Smith G, Stenhouse E, Crossley J, Aitken D, Cameron A, Connor J . Early pregnancy levels of pregnancy-associated plasma protein a and the risk of intrauterine growth restriction, premature birth, preeclampsia, and stillbirth. J Clin Endocrinol Metab. 2002; 87(4):1762-7. DOI: 10.1210/jcem.87.4.8430. View

5.
Imudia A, Suzuki Y, Kilburn B, Yelian F, Diamond M, Romero R . Retrieval of trophoblast cells from the cervical canal for prediction of abnormal pregnancy: a pilot study. Hum Reprod. 2009; 24(9):2086-92. PMC: 2727404. DOI: 10.1093/humrep/dep206. View