Utility of a Dengue-Derived Monoclonal Antibody to Enhance Zika Infection In Vitro

Overview

Journal PLoS Curr

Specialty General Medicine

Date 2016 Sep 24

PMID 27660733

Citations 31

Authors

Anu Susan Charles

Rebecca C Christofferson

Affiliations

Soon will be listed here.

Abstract

Introduction: Zika virus (ZIKV) has emerged in dengue (DENV) endemic areas, where these two related flaviviruses continue to co-circulate. DENV is a complex of four serotypes and infections can progress to severe disease. It is thought that this is mediated by antibody dependent enhancement (ADE) whereby antibodies from a primary DENV infection are incapable of neutralizing heterologous DENV infections with another serotype. ADE has been demonstrated among other members of the Flavivirus group.

Methods: We utilize an in vitro ADE assay developed for DENV to determine whether ZIKV is enhanced by a commonly available DENV serotype 2-derived monoclonal antibody (4G2).

Results: We show that ZIKV infection in vitro is enhanced in the presence of the 4G2 mAb.

Discussion: Our results demonstrate that ADE between ZIKV and DENV is possible and that the 4G2 antibody is a useful tool for the effects of pre-existing anti-DENV antibodies during ZIKV infections.

Citing Articles

Estimating per-infection cost and burden for dengue and Zika as a function of antibody-dependent enhancement.

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Comparative Analysis of In Vitro Models to Study Antibody-Dependent Enhancement of Zika Virus Infection.

Langerak T, Mumtaz N, Koopmans M, Schoenmakers S, Rockx B Viruses. 2022; 14(12).

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