Lateral Association and Elongation of Vimentin Intermediate Filament Proteins: A Time-resolved Light-scattering Study

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2016 Sep 23

PMID 27655889

Citations 14

Authors

Carlos G Lopez

Oliva Saldanha

Klaus Huber

Sarah Koster

Affiliations

Soon will be listed here.

Abstract

Vimentin intermediate filaments (IFs) are part of a family of proteins that constitute one of the three filament systems in the cytoskeleton, a major contributor to cell mechanics. One property that distinguishes IFs from the other cytoskeletal filament types, actin filaments and microtubules, is their highly hierarchical assembly pathway, where a lateral association step is followed by elongation. Here we present an innovative technique to follow the elongation reaction in solution and in situ by time-resolved static and dynamic light scattering, thereby precisely capturing the relevant time and length scales of seconds to minutes and 60-600 nm, respectively. We apply a quantitative model to our data and succeed in consistently describing the entire set of data, including particle mass, radius of gyration, and hydrodynamic radius during longitudinal association.

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