Light-Activated Triazabutadienes for the Modification of a Viral Surface

Overview

Journal Chembiochem

Specialty Biochemistry

Date 2016 Sep 21

PMID 27647786

Citations 7

Authors

Stephanie M Jensen

Flora W Kimani

John C Jewett

Affiliations

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Abstract

Chemical crosslinking is a versatile tool for the examination of biochemical interactions, in particular host-pathogen interactions. We report the critical first step toward the goal of probing these interactions by the synthesis and use of a new heterobifunctional crosslinker containing a triazabutadiene scaffold. The triazabutadiene is stable to protein conjugation and liberates a reactive aryl diazonium species upon irradiation with 350 nm light. We highlight the use of this technology by modifying the surface of several proteins, including the dengue virus envelope protein.

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