Successful Transplantation of Retinal Pigment Epithelial Cells from MHC Homozygote IPSCs in MHC-Matched Models

Overview

Journal Stem Cell Reports

Publisher Cell Press

Specialty Cell Biology

Date 2016 Sep 20

PMID 27641649

Citations 78

Authors

Sunao Sugita

Yuko Iwasaki

Kenichi Makabe

Hiroyuki Kamao

Michiko Mandai

Takashi Shiina

Kazumasa Ogasawara

Yasuhiko Hirami

Yasuo Kurimoto

Masayo Takahashi

Affiliations

Soon will be listed here.

Abstract

There is an ongoing controversy as to whether major histocompatibility complex (MHC) matching is a solution for allogeneic stem cell transplantation. In the present study, we established retinal pigment epithelial (RPE) cells from induced pluripotent stem cells (iPSCs) in MHC homozygote donors. We observed no rejection signs in iPSC-derived RPE allografts of MHC-matched animal models without immunosuppression, whereas there were immune attacks around the graft and retinal tissue damage in MHC-mismatched models. In an immunohistochemical examination of MHC-mismatched allografts, the transplanted RPE sheets/cells were located in the subretinal space, but the RPE exhibited inflammatory and hypertrophic changes, and many inflammatory cells, e.g., Iba1 cells, MHC class II cells, and CD3 T cells, invaded the graft area. Conversely, these inflammatory cells poorly infiltrated the area around the transplanted retina if MHC-matched allografts were used. Thus, cells derived from MHC homozygous donors could be used to treat retinal diseases in histocompatible recipients.

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