Different Dehydrins Perform Separate Functions in Physcomitrella Patens

Overview

Journal Planta

Specialty Biology

Date 2016 Sep 18

PMID 27638172

Citations 15

Authors

Tanushree Agarwal

Gouranga Upadhyaya

Tanmoy Halder

Abhishek Mukherjee

Arun Lahiri Majumder

Sudipta Ray

Affiliations

Soon will be listed here.

Abstract

Dehydrins, PpDHNA and PpDHNB from Physcomitrella patens provide drought and cold tolerance while PpDHNC shows antimicrobial property suggesting different dehydrins perform separate functions in P. patens. The moss Physcomitrella patens can withstand extremes of environmental condition including abiotic stress such as dehydration, salinity, low temperature and biotic stress such as pathogen attack. Osmotic stress is inflicted under both cold and drought stress conditions where dehydrins have been found to play a significant protective role. In this study, a comparative analysis was drawn for the three dehydrins PpDHNA, PpDHNB and PpDHNC from P. patens. Our data shows that PpDHNA and PpDHNB play a major role in cellular protection during osmotic stress. PpDHNB showed several fold upregulation of the gene when P. patens was subjected to cold and osmotic stress in combination. PpDHNA and PpDHNB provide protection to enzyme lactate dehydrogenase under osmotic as well as freezing conditions. PpDHNC possesses antibacterial activity and thus may have a role in biotic stress response. Overexpression of PpDHNA, PpDHNB and PpDHNC in transgenic tobacco showed a better performance for PpDHNB with respect to cold and osmotic stress. These results suggest that specific dehydrins contribute to tolerance of mosses under different stress conditions.

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References

Rahman L, Smith G, Bamm V, Voyer-Grant J, Moffatt B, Dutcher J . Phosphorylation of Thellungiella salsuginea dehydrins TsDHN-1 and TsDHN-2 facilitates cation-induced conformational changes and actin assembly. Biochemistry. 2011; 50(44):9587-604. DOI: 10.1021/bi201205m. View

Arnon D . COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. Plant Physiol. 1949; 24(1):1-15. PMC: 437905. DOI: 10.1104/pp.24.1.1. View

Hughes S, Schart V, Malcolmson J, Hogarth K, Martynowicz D, Tralman-Baker E . The importance of size and disorder in the cryoprotective effects of dehydrins. Plant Physiol. 2013; 163(3):1376-86. PMC: 3813657. DOI: 10.1104/pp.113.226803. View

Fischer E, Hansen B, Nair V, Hoyt F, Dorward D . Scanning electron microscopy. Curr Protoc Microbiol. 2012; Chapter 2:Unit 2B.2.. PMC: 3352184. DOI: 10.1002/9780471729259.mc02b02s25. View

Houde M, Dallaire S, NDong D, Sarhan F . Overexpression of the acidic dehydrin WCOR410 improves freezing tolerance in transgenic strawberry leaves. Plant Biotechnol J. 2006; 2(5):381-7. DOI: 10.1111/j.1467-7652.2004.00082.x. View

Houde M, Daniel C, Lachapelle M, Allard F, Laliberte S, Sarhan F . Immunolocalization of freezing-tolerance-associated proteins in the cytoplasm and nucleoplasm of wheat crown tissues. Plant J. 1995; 8(4):583-93. DOI: 10.1046/j.1365-313x.1995.8040583.x. View

Wanner L, Junttila O . Cold-induced freezing tolerance in Arabidopsis. Plant Physiol. 1999; 120(2):391-400. PMC: 59277. DOI: 10.1104/pp.120.2.391. View

Zhai C, Lan J, Wang H, Li L, Cheng X, Liu G . Rice dehydrin K-segments have in vitro antibacterial activity. Biochemistry (Mosc). 2011; 76(6):645-50. DOI: 10.1134/S0006297911060046. View

Ruibal C, Salamo I, Carballo V, Castro A, Bentancor M, Borsani O . Differential contribution of individual dehydrin genes from Physcomitrella patens to salt and osmotic stress tolerance. Plant Sci. 2012; 190:89-102. DOI: 10.1016/j.plantsci.2012.03.009. View

10.

Nylander M, Svensson J, Palva E, Welin B . Stress-induced accumulation and tissue-specific localization of dehydrins in Arabidopsis thaliana. Plant Mol Biol. 2001; 45(3):263-79. DOI: 10.1023/a:1006469128280. View

11.

Lin C, Thomashow M . A cold-regulated Arabidopsis gene encodes a polypeptide having potent cryoprotective activity. Biochem Biophys Res Commun. 1992; 183(3):1103-8. DOI: 10.1016/s0006-291x(05)80304-3. View

12.

Uemura M, Warren G, Steponkus P . Freezing sensitivity in the sfr4 mutant of Arabidopsis is due to low sugar content and is manifested by loss of osmotic responsiveness. Plant Physiol. 2003; 131(4):1800-7. PMC: 166936. DOI: 10.1104/pp.102.013227. View

13.

Ndong C, Danyluk J, Wilson K, Pocock T, Huner N, Sarhan F . Cold-regulated cereal chloroplast late embryogenesis abundant-like proteins. Molecular characterization and functional analyses. Plant Physiol. 2002; 129(3):1368-81. PMC: 166530. DOI: 10.1104/pp.001925. View

14.

Roitsch T . Source-sink regulation by sugar and stress. Curr Opin Plant Biol. 1999; 2(3):198-206. DOI: 10.1016/S1369-5266(99)80036-3. View

15.

Saavedra L, Svensson J, Carballo V, Izmendi D, Welin B, Vidal S . A dehydrin gene in Physcomitrella patens is required for salt and osmotic stress tolerance. Plant J. 2005; 45(2):237-49. DOI: 10.1111/j.1365-313X.2005.02603.x. View

16.

Sanchez-Ballesta M, Rodrigo M, Lafuente M, Granell A, Zacarias L . Dehydrin from citrus, which confers in vitro dehydration and freezing protection activity, is constitutive and highly expressed in the flavedo of fruit but responsive to cold and water stress in leaves. J Agric Food Chem. 2004; 52(7):1950-7. DOI: 10.1021/jf035216+. View

17.

Xie C, Zhang R, Qu Y, Miao Z, Zhang Y, Shen X . Overexpression of MtCAS31 enhances drought tolerance in transgenic Arabidopsis by reducing stomatal density. New Phytol. 2012; 195(1):124-35. DOI: 10.1111/j.1469-8137.2012.04136.x. View

18.

Rinne , Kaikuranta , van Der Plas LH , van der Schoot C . Dehydrins in cold-acclimated apices of birch (Betula pubescens ehrh. ): production, localization and potential role in rescuing enzyme function during dehydration. Planta. 1999; 209(4):377-88. DOI: 10.1007/s004250050740. View

19.

Kosova K, Vitamvas P, Prasil I . Wheat and barley dehydrins under cold, drought, and salinity - what can LEA-II proteins tell us about plant stress response?. Front Plant Sci. 2014; 5:343. PMC: 4089117. DOI: 10.3389/fpls.2014.00343. View

20.

Rorat T, Szabala B, Grygorowicz W, Wojtowicz B, Yin Z, Rey P . Expression of SK3-type dehydrin in transporting organs is associated with cold acclimation in Solanum species. Planta. 2006; 224(1):205-21. DOI: 10.1007/s00425-005-0200-1. View