Systems Biology-opportunities and Challenges: the Application of Proteomics to Study the Cardiovascular Extracellular Matrix

Overview

Journal Cardiovasc Res

Specialty Cardiology & Vascular Diseases

Date 2016 Sep 17

PMID 27635058

Citations 14

Authors

Javier Barallobre-Barreiro

Marc Lynch

Xiaoke Yin

Manuel Mayr

Affiliations

Soon will be listed here.

Abstract

Systems biology approaches including proteomics are becoming more widely used in cardiovascular research. In this review article, we focus on the application of proteomics to the cardiac extracellular matrix (ECM). ECM remodelling is a hallmark of many cardiovascular diseases. Proteomic techniques using mass spectrometry (MS) provide a platform for the comprehensive analysis of ECM proteins without a priori assumptions. Proteomics overcomes various constraints inherent to conventional antibody detection. On the other hand, studies that use whole tissue lysates for proteomic analysis mask the identification of the less abundant ECM constituents. In this review, we first discuss decellularization-based methods that enrich for ECM proteins in cardiac tissue, and how targeted MS allows for accurate protein quantification. The second part of the review will focus on post-translational modifications including hydroxylation and glycosylation and on the release of matrix fragments with biological activity (matrikines), all of which can be interrogated by proteomic techniques.

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