Rigid Cooperation of Per1 and Per2 Proteins

Overview

Journal Sci Rep

Specialty Science

Date 2016 Sep 10

PMID 27609640

Citations 7

Authors

Hiroyuki Tamiya

Sumito Ogawa

Yasuyoshi Ouchi

Masahiro Akishita

Affiliations

Soon will be listed here.

Abstract

Period circadian clock (Per) genes Per1 and Per2 have essential roles in circadian oscillation. In this study, we identified a new role of Per1-Per2 cooperation, and its mechanism, using our new experimental methods. Under constant light conditions, the period length of Per1 and Per2 knockout mice depended on the copy number ratio of Per1:Per2. We then established a light-emitting diode-based lighting system that can generate any pattern of light intensity. Under gradually changing light in the absence of phase shift with different periods, both Per1((-/-)) and Per2((-/-)) mice were entrained to a broader range of period length than wild-type mice. To analyse Per1-Per2 cooperative roles at the cell culture level, we established a Per2 knockout-rescue system, which can detect period shortening in a familial advanced sleep phase syndrome (FASPS) mutant. Upon introduction of the Per1 coding region in this system, we saw period shortening. In conclusion, short period-associated protein Per1 and long period-associated Per2 cooperated to rigidly confine the circadian period to "circa" 24-h. These results suggest that the rigid circadian rhythm maintained through the cooperation of Per1-Per2 could negatively impact modern society, in which the use of artificial lighting is ubiquitous, and result in circadian disorders, including delirium.

Citing Articles

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References

Jud C, Schmutz I, Hampp G, Oster H, Albrecht U . A guideline for analyzing circadian wheel-running behavior in rodents under different lighting conditions. Biol Proced Online. 2005; 7:101-16. PMC: 1190381. DOI: 10.1251/bpo109. View

Boulos Z, Macchi M, Terman M . Twilights widen the range of photic entrainment in hamsters. J Biol Rhythms. 2002; 17(4):353-63. DOI: 10.1177/074873002129002654. View

Meagher D, Moran M, Raju B, Gibbons D, Donnelly S, Saunders J . Phenomenology of delirium. Assessment of 100 adult cases using standardised measures. Br J Psychiatry. 2007; 190:135-41. DOI: 10.1192/bjp.bp.106.023911. View

Bae K, Jin X, Maywood E, Hastings M, Reppert S, Weaver D . Differential functions of mPer1, mPer2, and mPer3 in the SCN circadian clock. Neuron. 2001; 30(2):525-36. DOI: 10.1016/s0896-6273(01)00302-6. View

Vanselow K, Kramer A . Role of phosphorylation in the mammalian circadian clock. Cold Spring Harb Symp Quant Biol. 2008; 72:167-76. DOI: 10.1101/sqb.2007.72.036. View

Hatori M, Gill S, Mure L, Goulding M, OLeary D, Panda S . Lhx1 maintains synchrony among circadian oscillator neurons of the SCN. Elife. 2014; 3:e03357. PMC: 4137275. DOI: 10.7554/eLife.03357. View

Tamiya H, Hagino N, Okamoto A, Yamamoto K . Morning granulocytopenia in two patients with connective-tissue disease treated with oral corticosteroids. BMJ Case Rep. 2012; 2011. PMC: 3070373. DOI: 10.1136/bcr.12.2010.3622. View

Ukai H, Ueda H . Systems biology of mammalian circadian clocks. Annu Rev Physiol. 2010; 72:579-603. DOI: 10.1146/annurev-physiol-073109-130051. View

de Lange E, Verhaak P, van der Meer K . Prevalence, presentation and prognosis of delirium in older people in the population, at home and in long term care: a review. Int J Geriatr Psychiatry. 2012; 28(2):127-34. DOI: 10.1002/gps.3814. View

10.

Shanware N, Hutchinson J, Kim S, Zhan L, Bowler M, Tibbetts R . Casein kinase 1-dependent phosphorylation of familial advanced sleep phase syndrome-associated residues controls PERIOD 2 stability. J Biol Chem. 2011; 286(14):12766-74. PMC: 3069476. DOI: 10.1074/jbc.M111.224014. View

11.

Fong T, Tulebaev S, Inouye S . Delirium in elderly adults: diagnosis, prevention and treatment. Nat Rev Neurol. 2009; 5(4):210-20. PMC: 3065676. DOI: 10.1038/nrneurol.2009.24. View

12.

Isojima Y, Nakajima M, Ukai H, Fujishima H, Yamada R, Masumoto K . CKIepsilon/delta-dependent phosphorylation is a temperature-insensitive, period-determining process in the mammalian circadian clock. Proc Natl Acad Sci U S A. 2009; 106(37):15744-9. PMC: 2736905. DOI: 10.1073/pnas.0908733106. View

13.

Usui S, Takahashi Y, Okazaki T . Range of entrainment of rat circadian rhythms to sinusoidal light-intensity cycles. Am J Physiol Regul Integr Comp Physiol. 2000; 278(5):R1148-56. DOI: 10.1152/ajpregu.2000.278.5.R1148. View

14.

Czeisler C, Coleman R . Rotating shift work schedules that disrupt sleep are improved by applying circadian principles. Science. 1982; 217(4558):460-3. DOI: 10.1126/science.7089576. View

15.

Numano R, Yamazaki S, Umeda N, Samura T, Sujino M, Takahashi R . Constitutive expression of the Period1 gene impairs behavioral and molecular circadian rhythms. Proc Natl Acad Sci U S A. 2006; 103(10):3716-21. PMC: 1450145. DOI: 10.1073/pnas.0600060103. View

16.

Xu Y, Toh K, Jones C, Shin J, Fu Y, Ptacek L . Modeling of a human circadian mutation yields insights into clock regulation by PER2. Cell. 2007; 128(1):59-70. PMC: 1828903. DOI: 10.1016/j.cell.2006.11.043. View

17.

Albrecht U, Sun Z, Eichele G, Lee C . A differential response of two putative mammalian circadian regulators, mper1 and mper2, to light. Cell. 1998; 91(7):1055-64. DOI: 10.1016/s0092-8674(00)80495-x. View

18.

Friedrich G, Soriano P . Promoter traps in embryonic stem cells: a genetic screen to identify and mutate developmental genes in mice. Genes Dev. 1991; 5(9):1513-23. DOI: 10.1101/gad.5.9.1513. View

19.

Hirano A, Yumimoto K, Tsunematsu R, Matsumoto M, Oyama M, Kozuka-Hata H . FBXL21 regulates oscillation of the circadian clock through ubiquitination and stabilization of cryptochromes. Cell. 2013; 152(5):1106-18. DOI: 10.1016/j.cell.2013.01.054. View

20.

Gibbs J, Ince L, Matthews L, Mei J, Bell T, Yang N . An epithelial circadian clock controls pulmonary inflammation and glucocorticoid action. Nat Med. 2014; 20(8):919-26. PMC: 4268501. DOI: 10.1038/nm.3599. View