Amphotericin B Inhibits Enterovirus 71 Replication by Impeding Viral Entry

Overview

Journal Sci Rep

Specialty Science

Date 2016 Sep 10

PMID 27608771

Citations 8

Authors

Fengwen Xu

Xiaoxiao Zhao

Siqi Hu

Jian Li

Lijuan Yin

Shan Mei

Tingting Liu

Ying Wang

Lili Ren

Shan Cen

Zhendong Zhao

Jianwei Wang

Qi Jin

Chen Liang

Bin Ai

Fei Guo

Affiliations

Soon will be listed here.

Abstract

Enterovirus 71 (EV71) infection causes hand-foot-and-mouth disease that leads to cardiopulmonary complications and death in young children. There is thus an urgent need to find new treatments to control EV71 infection. In this study, we report potent inhibition of EV71 by a polyene antibiotic Amphotericin B. Amphotericin B profoundly diminished the expression of EV71 RNA and viral proteins in the RD cells and the HEK293 cells. As a result, EV71 production was inhibited by Amphotericin B with an EC50 (50% effective concentration) of 1.75 μM in RD cells and 0.32 μM in 293 cells. In addition to EV71, EV68 was also strongly inhibited by Amphotericin B. Results of mechanistic studies revealed that Amphotericin B targeted the early stage of EV71 infection through impairing the attachment and internalization of EV71 by host cells. As an effective anti-fungi drug, Amphotericin B thus holds the promise of formulating a novel therapeutic to treat EV71 infection.

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