Testing a Microarray to Detect and Monitor Toxic Microalgae in Arcachon Bay in France

Overview

Journal Microarrays (Basel)

Date 2016 Sep 9

PMID 27605178

Citations 4

Authors

Jessica U Kegel

Yolanda Del Amo

Laurence Costes

Linda K Medlin

Affiliations

Soon will be listed here.

Abstract

Harmful algal blooms (HABs) occur worldwide, causing health problems and economic damages to fisheries and tourism. Monitoring agencies are therefore essential, yet monitoring is based only on time-consuming light microscopy, a level at which a correct identification can be limited by insufficient morphological characters. The project MIDTAL (Microarray Detection of Toxic Algae)-an FP7-funded EU project-used rRNA genes (SSU and LSU) as a target on microarrays to identify toxic species. Furthermore, toxins were detected with a newly developed multiplex optical Surface Plasmon Resonance biosensor (Multi SPR) and compared with an enzyme-linked immunosorbent assay (ELISA). In this study, we demonstrate the latest generation of MIDTAL microarrays (version 3) and show the correlation between cell counts, detected toxin and microarray signals from field samples taken in Arcachon Bay in France in 2011. The MIDTAL microarray always detected more potentially toxic species than those detected by microscopic counts. The toxin detection was even more sensitive than both methods. Because of the universal nature of both toxin and species microarrays, they can be used to detect invasive species. Nevertheless, the MIDTAL microarray is not completely universal: first, because not all toxic species are on the chip, and second, because invasive species, such as Ostreopsis, already influence European coasts.

Citing Articles

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Molecular Techniques for the Detection of Organisms in Aquatic Environments, with Emphasis on Harmful Algal Bloom Species.

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Detection of emerging and re-emerging pathogens in surface waters close to an urban area.

Marcheggiani S, DUgo E, Puccinelli C, Giuseppetti R, DAngelo A, Gualerzi C Int J Environ Res Public Health. 2015; 12(5):5505-27.

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Evolution of the MIDTAL microarray: the adaption and testing of oligonucleotide 18S and 28S rDNA probes and evaluation of subsequent microarray generations with Prymnesium spp. cultures and field samples.

McCoy G, Touzet N, Fleming G, Raine R Environ Sci Pollut Res Int. 2015; 22(13):9704-16.

PMID: 25631743 DOI: 10.1007/s11356-015-4152-0.

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