Caldesmon and the Structure of Smooth Muscle Thin Filaments: Immunolocalization of Caldesmon on Thin Filaments

Overview

Journal J Muscle Res Cell Motil

Specialties Cell Biology
Physiology

Date 1989 Apr 1

PMID 2760189

Citations 18

Authors

W LEHMAN

R Craig

J Lui

C Moody

Affiliations

Soon will be listed here.

Abstract

Antibodies reacting with chicken gizzard caldesmon were used to determine the distribution of caldesmon on smooth muscle thin filaments. Antibodies developed against both the intact caldesmon molecule and a 40 kilodalton proteolytic fragment cause thin filaments to aggregate laterally. Aggregates produced with the latter antibody display regular periodic labelling with a repeat of approximately 38 nm, a distribution characteristic of proteins associated with tropomyosin on thin filaments. The stoichiometry of caldesmon on thin filaments has been critically reevaluated and alternative models of caldesmon distribution on thin filaments are proposed.

Citing Articles

Caldesmon and the regulation of cytoskeletal functions.

Wang C Adv Exp Med Biol. 2009; 644:250-72.

PMID: 19209827 PMC: 2975104. DOI: 10.1007/978-0-387-85766-4_19.

Modulation of actin mechanics by caldesmon and tropomyosin.

Greenberg M, Wang C, LEHMAN W, Moore J Cell Motil Cytoskeleton. 2007; 65(2):156-64.

PMID: 18000881 PMC: 2975105. DOI: 10.1002/cm.20251.

Caldesmon exhibits a clustered distribution along individual chicken gizzard native thin filaments.

Mabuchi K, Li Y, Carlos A, Wang C, Graceffa P J Muscle Res Cell Motil. 2001; 22(1):77-90.

PMID: 11563552 DOI: 10.1023/a:1010392322503.

Sarcomeric binding pattern of exogenously added intact caldesmon and its C-terminal 20-kDa fragment in skinned fibers of skeletal muscle.

Frisbie S, Reedy M, Yu L, Brenner B, Chalovich J, Kraft T J Muscle Res Cell Motil. 1999; 20(3):291-303.

PMID: 10471992 DOI: 10.1023/a:1005490405222.

Three-dimensional image reconstruction of reconstituted smooth muscle thin filaments: effects of caldesmon.

Hodgkinson J, Marston S, Craig R, Vibert P, LEHMAN W Biophys J. 1997; 72(6):2398-404.

PMID: 9168017 PMC: 1184439. DOI: 10.1016/S0006-3495(97)78885-4.

References

OTSUKI I, Masaki T, Nonomura Y, Ebashi S . Periodic distribution of troponin along the thin filament. J Biochem. 1967; 61(6):817-9. DOI: 10.1093/oxfordjournals.jbchem.a128619. View

Valentine R, Green N . Electron microscopy of an antibody-hapten complex. J Mol Biol. 1967; 27(3):615-7. DOI: 10.1016/0022-2836(67)90063-0. View

Bagby R . Toward a comprehensive three-dimensional model of the contractile system of vertebrate smooth muscle cells. Int Rev Cytol. 1986; 105:67-128. DOI: 10.1016/s0074-7696(08)61062-1. View

Szpacenko A, Dabrowska R . Functional domain of caldesmon. FEBS Lett. 1986; 202(2):182-6. DOI: 10.1016/0014-5793(86)80683-4. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Lynch W, Riseman V, BRETSCHER A . Smooth muscle caldesmon is an extended flexible monomeric protein in solution that can readily undergo reversible intra- and intermolecular sulfhydryl cross-linking. A mechanism for caldesmon's F-actin bundling activity. J Biol Chem. 1987; 262(15):7429-37. View

Taylor D, Reidler J, Spudich J, Stryer L . Detection of actin assembly by fluorescence energy transfer. J Cell Biol. 1981; 89(2):362-7. PMC: 2111691. DOI: 10.1083/jcb.89.2.362. View

Elzinga M, COLLINS J, Kuehl W, Adelstein R . Complete amino-acid sequence of actin of rabbit skeletal muscle. Proc Natl Acad Sci U S A. 1973; 70(9):2687-91. PMC: 427084. DOI: 10.1073/pnas.70.9.2687. View

Clark T, Ngai P, Sutherland C, Walsh M . Vascular smooth muscle caldesmon. J Biol Chem. 1986; 261(17):8028-35. View

10.

Ngai P, Walsh M . Inhibition of smooth muscle actin-activated myosin Mg2+-ATPase activity by caldesmon. J Biol Chem. 1984; 259(22):13656-9. View

11.

LEHMAN W, Sheldon A, Madonia W . Diversity in smooth muscle thin filament composition. Biochim Biophys Acta. 1987; 914(1):35-9. DOI: 10.1016/0167-4838(87)90158-0. View

12.

Chacko S, Conti M, Adelstein R . Effect of phosphorylation of smooth muscle myosin on actin activation and Ca2+ regulation. Proc Natl Acad Sci U S A. 1977; 74(1):129-33. PMC: 393211. DOI: 10.1073/pnas.74.1.129. View

13.

Lash J, Sellers J, Hathaway D . The effects of caldesmon on smooth muscle heavy actomeromyosin ATPase activity and binding of heavy meromyosin to actin. J Biol Chem. 1986; 261(34):16155-60. View

14.

Fujii T, Imai M, Rosenfeld G, Bryan J . Domain mapping of chicken gizzard caldesmon. J Biol Chem. 1987; 262(6):2757-63. View

15.

SOBER H, Peterson E . Protein chromatography on ion exchange cellulose. Fed Proc. 1958; 17(4):1116-26. View

16.

Graceffa P . Evidence for interaction between smooth muscle tropomyosin and caldesmon. FEBS Lett. 1987; 218(1):139-42. DOI: 10.1016/0014-5793(87)81034-7. View

17.

Gorecka A, Aksoy M, Hartshorne D . The effect of phosphorylation of gizzard myosin on actin activation. Biochem Biophys Res Commun. 1976; 71(1):325-31. DOI: 10.1016/0006-291x(76)90286-2. View

18.

Towbin H, Staehelin T, Gordon J . Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979; 76(9):4350-4. PMC: 411572. DOI: 10.1073/pnas.76.9.4350. View

19.

Sarma V, Silverton E, Davies D, TERRY W . The three-dimensional structure at 6 A resolution of a human gamma Gl immunoglobulin molecule. J Biol Chem. 1971; 246(11):3753-9. View

20.

Chalovich J, Cornelius P, Benson C . Caldesmon inhibits skeletal actomyosin subfragment-1 ATPase activity and the binding of myosin subfragment-1 to actin. J Biol Chem. 1987; 262(12):5711-6. View