Sugarcane Serine Peptidase Inhibitors, Serine Peptidases, and Clp Protease System Subunits Associated with Sugarcane Borer (Diatraea Saccharalis) Herbivory and Wounding

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2016 Sep 7

PMID 27598134

Citations 2

Authors

Ane H Medeiros

Fabiana B Mingossi

Renata O Dias

Flavia P Franco

Renato Vicentini

Marcia O Mello

Daniel S Moura

Marcio C Silva-Filho

Affiliations

Soon will be listed here.

Abstract

Sugarcane's (Saccharum spp.) response to Diatraea saccharalis (F.) (Lepidoptera: (Crambidae) herbivory was investigated using a macroarray spotted with 248 sugarcane Expressed Sequence Tags (ESTs) encoding serine peptidase inhibitors, serine peptidases. and Clp protease system subunits. Our results showed that after nine hours of herbivory, 13 sugarcane genes were upregulated and nine were downregulated. Among the upregulated genes, nine were similar to serine peptidase inhibitors and four were similar to Bowman-Birk Inhibitors (BBIs). Phylogenetic analysis revealed that these sequences belong to a phylogenetic group of sugarcane BBIs that are potentially involved in plant defense against insect predation. The remaining four upregulated genes included serine peptidases and one homolog to the Arabidopsis AAA+ chaperone subunit ClpD, which is a member of the Clp protease system. Among the downregulated genes, five were homologous to serine peptidases and four were homologous to Arabidopsis Clp subunits (three homologous to Clp AAA+ chaperones and one to a ClpP-related ClpR subunit). Although the roles of serine peptidase inhibitors in plant defenses against herbivory have been extensively investigated, the roles of plant serine peptidases and the Clp protease system represent a new and underexplored field of study. The up- and downregulated D. saccharalis genes presented in this study may be candidate genes for the further investigation of the sugarcane response to herbivory.

Citing Articles

Structural and Functional Characterization of PR-4 SUGARWINs From Sugarcaneand Their Role in Plant Defense.

Franco F, Dias R, Toyama D, Henrique-Silva F, S Moura D, Silva-Filho M Front Plant Sci. 2019; 9:1916.

PMID: 30666261 PMC: 6330325. DOI: 10.3389/fpls.2018.01916.

Plant Bio-Wars: Maize Protein Networks Reveal Tissue-Specific Defense Strategies in Response to a Root Herbivore.

Castano-Duque L, Helms A, Ali J, Luthe D J Chem Ecol. 2018; 44(7-8):727-745.

PMID: 29926336 DOI: 10.1007/s10886-018-0972-y.

References

Mithofer A, Boland W . Recognition of herbivory-associated molecular patterns. Plant Physiol. 2008; 146(3):825-31. PMC: 2259064. DOI: 10.1104/pp.107.113118. View

Moura D, Bergey D, Ryan C . Characterization and localization of a wound-inducible type I serine-carboxypeptidase from leaves of tomato plants (Lycopersicon esculentum Mill.). Planta. 2001; 212(2):222-30. DOI: 10.1007/s004250000380. View

Cordero M, Raventos D, San Segundo B . Expression of a maize proteinase inhibitor gene is induced in response to wounding and fungal infection: systemic wound-response of a monocot gene. Plant J. 1994; 6(2):141-50. DOI: 10.1046/j.1365-313x.1994.6020141.x. View

Botella M, Xu Y, Prabha T, Zhao Y, Narasimhan M, Wilson K . Differential expression of soybean cysteine proteinase inhibitor genes during development and in response to wounding and methyl jasmonate. Plant Physiol. 1996; 112(3):1201-10. PMC: 158047. DOI: 10.1104/pp.112.3.1201. View

Maheswaran G, Pridmore L, Franz P, Anderson M . A proteinase inhibitor from Nicotiana alata inhibits the normal development of light-brown apple moth, Epiphyas postvittana in transgenic apple plants. Plant Cell Rep. 2007; 26(6):773-82. DOI: 10.1007/s00299-006-0281-8. View

Pautot V, Holzer F, Chaufaux J, Walling L . The induction of tomato leucine aminopeptidase genes (LapA) after Pseudomonas syringae pv. tomato infection is primarily a wound response triggered by coronatine. Mol Plant Microbe Interact. 2001; 14(2):214-24. DOI: 10.1094/MPMI.2001.14.2.214. View

Farag M, Fokar M, Abd H, Zhang H, Allen R, Pare P . (Z)-3-Hexenol induces defense genes and downstream metabolites in maize. Planta. 2004; 220(6):900-9. DOI: 10.1007/s00425-004-1404-5. View

Vettore A, da Silva F, Kemper E, Souza G, da Silva A, Ferro M . Analysis and functional annotation of an expressed sequence tag collection for tropical crop sugarcane. Genome Res. 2003; 13(12):2725-35. PMC: 403815. DOI: 10.1101/gr.1532103. View

Ryan C, S Moura D . Systemic wound signaling in plants: a new perception. Proc Natl Acad Sci U S A. 2002; 99(10):6519-20. PMC: 124432. DOI: 10.1073/pnas.112196499. View

10.

Pfaffl M, Horgan G, Dempfle L . Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acids Res. 2002; 30(9):e36. PMC: 113859. DOI: 10.1093/nar/30.9.e36. View

11.

Rocha F, Papini-Terzi F, Nishiyama Jr M, Vencio R, Vicentini R, Duarte R . Signal transduction-related responses to phytohormones and environmental challenges in sugarcane. BMC Genomics. 2007; 8:71. PMC: 1852312. DOI: 10.1186/1471-2164-8-71. View

12.

Wei F, Wing R, Wise R . Genome dynamics and evolution of the Mla (powdery mildew) resistance locus in barley. Plant Cell. 2002; 14(8):1903-17. PMC: 151473. DOI: 10.1105/tpc.002238. View

13.

Birk Y, Gertler A, KHALEF S . A pure trypsin inhibitor from soya beans. Biochem J. 1963; 87:281-4. PMC: 1201889. DOI: 10.1042/bj0870281. View

14.

Howe G, Lightner J, Browse J, Ryan C . An octadecanoid pathway mutant (JL5) of tomato is compromised in signaling for defense against insect attack. Plant Cell. 1996; 8(11):2067-77. PMC: 161335. DOI: 10.1105/tpc.8.11.2067. View

15.

Schnable P, Ware D, Fulton R, Stein J, Wei F, Pasternak S . The B73 maize genome: complexity, diversity, and dynamics. Science. 2009; 326(5956):1112-5. DOI: 10.1126/science.1178534. View

16.

Chinault A, Sternberg N . Screening large-insert libraries by PCR. Curr Protoc Hum Genet. 2008; Chapter 5:Unit 5.5. DOI: 10.1002/0471142905.hg0505s00. View

17.

Tamayo M, Rufat M, Bravo J, San Segundo B . Accumulation of a maize proteinase inhibitor in response to wounding and insect feeding, and characterization of its activity toward digestive proteinases of Spodoptera littoralis larvae. Planta. 2000; 211(1):62-71. DOI: 10.1007/s004250000258. View

18.

Menossi M, Silva-Filho M, Vincentz M, Van-Sluys M, Souza G . Sugarcane functional genomics: gene discovery for agronomic trait development. Int J Plant Genomics. 2008; 2008:458732. PMC: 2216073. DOI: 10.1155/2008/458732. View

19.

Fowler J, Narvaez-Vasquez J, Aromdee D, Pautot V, Holzer F, Walling L . Leucine aminopeptidase regulates defense and wound signaling in tomato downstream of jasmonic acid. Plant Cell. 2009; 21(4):1239-51. PMC: 2685619. DOI: 10.1105/tpc.108.065029. View

20.

Porankiewicz J, Schelin J, Clarke A . The ATP-dependent Clp protease is essential for acclimation to UV-B and low temperature in the cyanobacterium Synechococcus. Mol Microbiol. 1998; 29(1):275-83. DOI: 10.1046/j.1365-2958.1998.00928.x. View