HSAF-induced Antifungal Effects in Through ROS-mediated Apoptosis

Overview

Journal RSC Adv

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2016 Sep 6

PMID 27594989

Citations 24

Authors

Yanjiao Ding

Zhenyu Li

Yaoyao Li

Chunhua Lu

Haoxin Wang

Yuemao Shen

Liangcheng Du

Affiliations

Soon will be listed here.

Abstract

Heat-stable antifungal factor (HSAF) belongs to polycyclic tetramate macrolactams (PTMs), which inhibits many fungal pathogens and is effective in inhibiting s (). In this study, we found that HSAF induced the apoptosis of s SC5314 through inducing the production of reactive oxygen species (ROS). Nevertheless, we validated the efficacy of HSAF against candidiasis caused by in a murine model in vivo, and HSAF significantly improved survival and reduced fungal burden compared to vehicles. A molecular dynamics (MD) simulation was also investigated, revealing the theoretical binding mode of HSAF to the β-tubulin of . This study first found PTMs-induced fungal apoptosis through ROS accumulation in and its potential as a novel agent for fungicides.

Citing Articles

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Developing a new treatment for superficial fungal infection using antifungal Collagen-HSAF dressing.

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Polyene Macrolactams from Marine and Terrestrial Sources: Structure, Production Strategies, Biosynthesis and Bioactivities.

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References

Keren I, Wu Y, Inocencio J, Mulcahy L, Lewis K . Killing by bactericidal antibiotics does not depend on reactive oxygen species. Science. 2013; 339(6124):1213-6. DOI: 10.1126/science.1232688. View

Lou L, Chen H, Cerny R, Li Y, Shen Y, Du L . Unusual activities of the thioesterase domain for the biosynthesis of the polycyclic tetramate macrolactam HSAF in Lysobacter enzymogenes C3. Biochemistry. 2011; 51(1):4-6. PMC: 3258519. DOI: 10.1021/bi2015025. View

Guex N, Peitsch M, Schwede T . Automated comparative protein structure modeling with SWISS-MODEL and Swiss-PdbViewer: a historical perspective. Electrophoresis. 2009; 30 Suppl 1:S162-73. DOI: 10.1002/elps.200900140. View

Belenky P, Camacho D, Collins J . Fungicidal drugs induce a common oxidative-damage cellular death pathway. Cell Rep. 2013; 3(2):350-8. PMC: 3656588. DOI: 10.1016/j.celrep.2012.12.021. View

Kiefer F, Arnold K, Kunzli M, Bordoli L, Schwede T . The SWISS-MODEL Repository and associated resources. Nucleic Acids Res. 2008; 37(Database issue):D387-92. PMC: 2686475. DOI: 10.1093/nar/gkn750. View

Kohanski M, Dwyer D, Wierzbowski J, Cottarel G, Collins J . Mistranslation of membrane proteins and two-component system activation trigger antibiotic-mediated cell death. Cell. 2008; 135(4):679-90. PMC: 2684502. DOI: 10.1016/j.cell.2008.09.038. View

Biasini M, Bienert S, Waterhouse A, Arnold K, Studer G, Schmidt T . SWISS-MODEL: modelling protein tertiary and quaternary structure using evolutionary information. Nucleic Acids Res. 2014; 42(Web Server issue):W252-8. PMC: 4086089. DOI: 10.1093/nar/gku340. View

Cho J, Lee D . The antimicrobial peptide arenicin-1 promotes generation of reactive oxygen species and induction of apoptosis. Biochim Biophys Acta. 2011; 1810(12):1246-51. DOI: 10.1016/j.bbagen.2011.08.011. View

Liu H, Sun M, Du D, Pan H, Cheng T, Wang J . Whole-Transcriptome Analysis of Differentially Expressed Genes in the Vegetative Buds, Floral Buds and Buds of Chrysanthemum morifolium. PLoS One. 2015; 10(5):e0128009. PMC: 4444331. DOI: 10.1371/journal.pone.0128009. View

10.

Phillips A, Sudbery I, Ramsdale M . Apoptosis induced by environmental stresses and amphotericin B in Candida albicans. Proc Natl Acad Sci U S A. 2003; 100(24):14327-32. PMC: 283591. DOI: 10.1073/pnas.2332326100. View

11.

Hwang J, Choi H, Kim A, Yun J, Yu R, Woo E . Hibicuslide C-induced cell death in Candida albicans involves apoptosis mechanism. J Appl Microbiol. 2014; 117(5):1400-11. DOI: 10.1111/jam.12633. View

12.

Case D, Cheatham 3rd T, Darden T, Gohlke H, Luo R, Merz Jr K . The Amber biomolecular simulation programs. J Comput Chem. 2005; 26(16):1668-88. PMC: 1989667. DOI: 10.1002/jcc.20290. View

13.

Hof H . A new, broad-spectrum azole antifungal: posaconazole--mechanisms of action and resistance, spectrum of activity. Mycoses. 2006; 49 Suppl 1:2-6. DOI: 10.1111/j.1439-0507.2006.01295.x. View

14.

Lee W, Lee D . Magainin 2 induces bacterial cell death showing apoptotic properties. Curr Microbiol. 2014; 69(6):794-801. DOI: 10.1007/s00284-014-0657-x. View

15.

Shin K, Yoon Y, Kang Y, Son K, Kim H, Kwon B . KRIBB3, a novel microtubule inhibitor, induces mitotic arrest and apoptosis in human cancer cells. Biochem Pharmacol. 2007; 75(2):383-94. DOI: 10.1016/j.bcp.2007.08.027. View

16.

Zhu J, Krom B, Sanglard D, Intapa C, Dawson C, Peters B . Farnesol-induced apoptosis in Candida albicans is mediated by Cdr1-p extrusion and depletion of intracellular glutathione. PLoS One. 2011; 6(12):e28830. PMC: 3242750. DOI: 10.1371/journal.pone.0028830. View

17.

Mollinedo F, Gajate C . Microtubules, microtubule-interfering agents and apoptosis. Apoptosis. 2003; 8(5):413-50. DOI: 10.1023/a:1025513106330. View

18.

Baines C, Kaiser R, Purcell N, Blair N, Osinska H, Hambleton M . Loss of cyclophilin D reveals a critical role for mitochondrial permeability transition in cell death. Nature. 2005; 434(7033):658-62. DOI: 10.1038/nature03434. View

19.

Vanlangenakker N, Vanden Berghe T, Vandenabeele P . Many stimuli pull the necrotic trigger, an overview. Cell Death Differ. 2011; 19(1):75-86. PMC: 3252835. DOI: 10.1038/cdd.2011.164. View

20.

Weingartner A, Kemmer G, Muller F, Andrade Zampieri R, Gonzaga Dos Santos M, Schiller J . Leishmania promastigotes lack phosphatidylserine but bind annexin V upon permeabilization or miltefosine treatment. PLoS One. 2012; 7(8):e42070. PMC: 3411662. DOI: 10.1371/journal.pone.0042070. View