A New Micro Swim-up Procedure for Sperm Preparation in ICSI Treatments: Preliminary Microbiological Testing

Overview

Journal JBRA Assist Reprod

Specialty Reproductive Medicine

Date 2016 Sep 2

PMID 27584599

Citations 6

Authors

Simone Palini

Mariangela Primiterra

Silvia De Stefani

Maria Federica Pedna

Monica Sparacino

Patrizia Farabegoli

Serena Benedetti

Carlo Bulletti

Vittorio Sambri

Affiliations

Soon will be listed here.

Abstract

Objective: This study aimed to assess the levels of microbial contamination in semen samples before and after the micro swim-up (MSU) procedure in intra-cytoplasmic sperm injection (ICSI). The new method is an upgrade to the classic wash swim-up procedure.

Methods: Semen analysis and microbiological tests were carried out before and after the MSU procedure. A total of twenty semen samples were analyzed.

Results: Pathogens were observed in semen samples only before MSU and never after ICSI. Microbiological tests revealed a large prevalence of gram-positive cocci [Staphylococcus spp. (n=16, 80%) and viridans streptococci (n=10, 50%)]. The results of this study indicate that direct MSU in ICSI improved the ICSI workflow.

Conclusion: The new workflow is faster and more affordable, and is likely to prevent infection problems that could arise from the normal microbial flora of the semen.

Citing Articles

Next-Generation Sequencing to Elucidate the Semen Microbiome in Male Reproductive Disorders.

Davies R, Minhas S, Jayasena C Medicina (Kaunas). 2024; 60(1).

PMID: 38256286 PMC: 10819355. DOI: 10.3390/medicina60010025.

A fast and safe technique for sperm preparation in ICSI treatments within a randomized controlled trial (RCT).

Baldini D, Baldini A, Silvestris E, Vizziello G, Ferri D, Vizziello D Reprod Biol Endocrinol. 2020; 18(1):88.

PMID: 32814589 PMC: 7437070. DOI: 10.1186/s12958-020-00642-8.

The complex microbiome from native semen to embryo culture environment in human in vitro fertilization procedure.

Stsepetova J, Baranova J, Simm J, Parm U, Roop T, Sokmann S Reprod Biol Endocrinol. 2020; 18(1):3.

PMID: 31948459 PMC: 6966866. DOI: 10.1186/s12958-019-0562-z.

Microbial contamination in assisted reproductive technology: source, prevalence, and cost.

Borges E, Berteli T, Reis T, Silva A, Vireque A J Assist Reprod Genet. 2019; 37(1):53-61.

PMID: 31823133 PMC: 7000601. DOI: 10.1007/s10815-019-01640-5.

The seminal microbiome in health and disease.

Altmae S, Franasiak J, Mandar R Nat Rev Urol. 2019; 16(12):703-721.

PMID: 31732723 DOI: 10.1038/s41585-019-0250-y.

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