A Decade of Progress in Tissue Engineering

Overview

Journal Nat Protoc

Specialties Biology
Pathology
Science

Date 2016 Sep 2

PMID 27583639

Citations 223

Authors

Ali Khademhosseini

Robert Langer

Affiliations

Soon will be listed here.

Abstract

Tremendous progress has been achieved in the field of tissue engineering in the past decade. Several major challenges laid down 10 years ago, have been studied, including renewable cell sources, biomaterials with tunable properties, mitigation of host responses, and vascularization. Here we review advancements in these areas and envision directions of further development.

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References

Du Y, Ghodousi M, Lo E, Vidula M, Emiroglu O, Khademhosseini A . Surface-directed assembly of cell-laden microgels. Biotechnol Bioeng. 2009; 105(3):655-62. PMC: 2833321. DOI: 10.1002/bit.22552. View

Wilmer M, Ng C, Lanz H, Vulto P, Suter-Dick L, Masereeuw R . Kidney-on-a-Chip Technology for Drug-Induced Nephrotoxicity Screening. Trends Biotechnol. 2015; 34(2):156-170. DOI: 10.1016/j.tibtech.2015.11.001. View

Huebsch N, Arany P, Mao A, Shvartsman D, Ali O, Bencherif S . Harnessing traction-mediated manipulation of the cell/matrix interface to control stem-cell fate. Nat Mater. 2010; 9(6):518-26. PMC: 2919753. DOI: 10.1038/nmat2732. View

Menze M, Chakraborty N, Clavenna M, Banerjee M, Liu X, Toner M . Metabolic preconditioning of cells with AICAR-riboside: improved cryopreservation and cell-type specific impacts on energetics and proliferation. Cryobiology. 2010; 61(1):79-88. PMC: 2920351. DOI: 10.1016/j.cryobiol.2010.05.004. View

Zhang W, Zhang Y, Bakht S, Aleman J, Shin S, Yue K . Elastomeric free-form blood vessels for interconnecting organs on chip systems. Lab Chip. 2016; 16(9):1579-86. PMC: 4846563. DOI: 10.1039/c6lc00001k. View

Esch E, Bahinski A, Huh D . Organs-on-chips at the frontiers of drug discovery. Nat Rev Drug Discov. 2015; 14(4):248-60. PMC: 4826389. DOI: 10.1038/nrd4539. View

Pakulska M, Miersch S, Shoichet M . Designer protein delivery: From natural to engineered affinity-controlled release systems. Science. 2016; 351(6279):aac4750. DOI: 10.1126/science.aac4750. View

Wilson Jr W, Boland T . Cell and organ printing 1: protein and cell printers. Anat Rec A Discov Mol Cell Evol Biol. 2003; 272(2):491-6. DOI: 10.1002/ar.a.10057. View

Heo Y, Nagrath S, Moore A, Zeinali M, Irimia D, Stott S . "Universal" vitrification of cells by ultra-fast cooling. Technology (Singap World Sci). 2015; 3(1):64-71. PMC: 4404302. DOI: 10.1142/S2339547815500053. View

10.

ZuK P, Zhu M, Ashjian P, De Ugarte D, Huang J, Mizuno H . Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell. 2002; 13(12):4279-95. PMC: 138633. DOI: 10.1091/mbc.e02-02-0105. View

11.

Ranganath S, Levy O, Inamdar M, Karp J . Harnessing the mesenchymal stem cell secretome for the treatment of cardiovascular disease. Cell Stem Cell. 2012; 10(3):244-58. PMC: 3294273. DOI: 10.1016/j.stem.2012.02.005. View

12.

Sullivan D, Mirmalek-Sani S, Deegan D, Baptista P, Aboushwareb T, Atala A . Decellularization methods of porcine kidneys for whole organ engineering using a high-throughput system. Biomaterials. 2012; 33(31):7756-64. DOI: 10.1016/j.biomaterials.2012.07.023. View

13.

Azagarsamy M, Anseth K . Bioorthogonal Click Chemistry: An Indispensable Tool to Create Multifaceted Cell Culture Scaffolds. ACS Macro Lett. 2013; 2(1):5-9. PMC: 3547663. DOI: 10.1021/mz300585q. View

14.

Swift J, Ivanovska I, Buxboim A, Harada T, Dingal P, Pinter J . Nuclear lamin-A scales with tissue stiffness and enhances matrix-directed differentiation. Science. 2013; 341(6149):1240104. PMC: 3976548. DOI: 10.1126/science.1240104. View

15.

Christensen K, Xu C, Chai W, Zhang Z, Fu J, Huang Y . Freeform inkjet printing of cellular structures with bifurcations. Biotechnol Bioeng. 2014; 112(5):1047-55. DOI: 10.1002/bit.25501. View

16.

Langer R, Vacanti J . Tissue engineering. Science. 1993; 260(5110):920-6. DOI: 10.1126/science.8493529. View

17.

Murphy S, Atala A . 3D bioprinting of tissues and organs. Nat Biotechnol. 2014; 32(8):773-85. DOI: 10.1038/nbt.2958. View

18.

Khademhosseini A, Langer R . Microengineered hydrogels for tissue engineering. Biomaterials. 2007; 28(34):5087-92. DOI: 10.1016/j.biomaterials.2007.07.021. View

19.

Okita K, Ichisaka T, Yamanaka S . Generation of germline-competent induced pluripotent stem cells. Nature. 2007; 448(7151):313-7. DOI: 10.1038/nature05934. View

20.

Kaushal S, Amiel G, Guleserian K, Shapira O, Perry T, Sutherland F . Functional small-diameter neovessels created using endothelial progenitor cells expanded ex vivo. Nat Med. 2001; 7(9):1035-40. PMC: 2818999. DOI: 10.1038/nm0901-1035. View