A Wheat COP9 Subunit 5-like Gene is Negatively Involved in Host Response to Leaf Rust

Overview

Journal Mol Plant Pathol

Specialty Molecular Biology

Date 2016 Sep 2

PMID 27581057

Citations 8

Authors

Hongtao Zhang

Xiaojing Wang

Michael J Giroux

Li Huang

Affiliations

Soon will be listed here.

Abstract

The COP9 (constitutive photomorphogenesis 9) signalosome (CSN) is a protein complex involved in the ubiquitin proteasome system and a common host target of diverse pathogens in Arabidopsis. The known derubylation function of the COP9 complex is carried out by subunit 5 encoded by AtCSN5A or AtCSN5B in Arabidopsis. A single CSN5-like gene (designated as TaCSN5) with three homeologues was identified on the long arms of wheat (Triticum aestivum L.) group 2 chromosomes. In this study, we identified and characterized the function of TaCSN5 in response to infection by the leaf rust pathogen. Down-regulation of all three TaCSN5 homeologues or mutations in the homeologues on chromosomes 2A or 2D resulted in significantly enhanced resistance to leaf rust. Enhanced leaf rust resistance corresponded to a seven-fold increase in PR1 (pathogenesis-related gene 1) expression. Collectively, the data indicate that the wheat COP9 subunit 5-like gene acts as a negative regulator of wheat leaf rust resistance.

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References

Wei N, Deng X . Making sense of the COP9 signalosome. A regulatory protein complex conserved from Arabidopsis to human. Trends Genet. 1999; 15(3):98-103. DOI: 10.1016/s0168-9525(98)01670-9. View

Karniol B, Malec P, Chamovitz D . Arabidopsis FUSCA5 encodes a novel phosphoprotein that is a component of the COP9 complex. Plant Cell. 1999; 11(5):839-48. PMC: 144222. DOI: 10.1105/tpc.11.5.839. View

Freilich S, Oron E, KAPP Y, Orgad S, Segal D, Chamovitz D . The COP9 signalosome is essential for development of Drosophila melanogaster. Curr Biol. 1999; 9(20):1187-90. DOI: 10.1016/S0960-9822(00)80023-8. View

Mundt K, Porte J, Murray J, Brikos C, Christensen P, Caspari T . The COP9/signalosome complex is conserved in fission yeast and has a role in S phase. Curr Biol. 1999; 9(23):1427-30. DOI: 10.1016/s0960-9822(00)80091-3. View

Kloek A, Verbsky M, Sharma S, Schoelz J, Vogel J, Klessig D . Resistance to Pseudomonas syringae conferred by an Arabidopsis thaliana coronatine-insensitive (coi1) mutation occurs through two distinct mechanisms. Plant J. 2001; 26(5):509-22. DOI: 10.1046/j.1365-313x.2001.01050.x. View

Turner J, Ellis C, Devoto A . The jasmonate signal pathway. Plant Cell. 2002; 14 Suppl:S153-64. PMC: 151253. DOI: 10.1105/tpc.000679. View

Liu Y, Schiff M, Serino G, Deng X, Dinesh-Kumar S . Role of SCF ubiquitin-ligase and the COP9 signalosome in the N gene-mediated resistance response to Tobacco mosaic virus. Plant Cell. 2002; 14(7):1483-96. PMC: 150701. DOI: 10.1105/tpc.002493. View

Kunkel B, Brooks D . Cross talk between signaling pathways in pathogen defense. Curr Opin Plant Biol. 2002; 5(4):325-31. DOI: 10.1016/s1369-5266(02)00275-3. View

Ryals J, Neuenschwander U, Willits M, Molina A, Steiner H, HUNT M . Systemic Acquired Resistance. Plant Cell. 1996; 8(10):1809-1819. PMC: 161316. DOI: 10.1105/tpc.8.10.1809. View

10.

WARD E, Uknes S, Williams S, Dincher S, Wiederhold D, Alexander D . Coordinate Gene Activity in Response to Agents That Induce Systemic Acquired Resistance. Plant Cell. 1991; 3(10):1085-1094. PMC: 160074. DOI: 10.1105/tpc.3.10.1085. View

11.

Schwechheimer C, Serino G, Deng X . Multiple ubiquitin ligase-mediated processes require COP9 signalosome and AXR1 function. Plant Cell. 2002; 14(10):2553-63. PMC: 151235. DOI: 10.1105/tpc.003434. View

12.

Feng S, Ma L, Wang X, Xie D, Dinesh-Kumar S, Wei N . The COP9 signalosome interacts physically with SCF COI1 and modulates jasmonate responses. Plant Cell. 2003; 15(5):1083-94. PMC: 153718. DOI: 10.1105/tpc.010207. View

13.

Dodds P, Lawrence G, Catanzariti A, Ayliffe M, Ellis J . The Melampsora lini AvrL567 avirulence genes are expressed in haustoria and their products are recognized inside plant cells. Plant Cell. 2004; 16(3):755-68. PMC: 385286. DOI: 10.1105/tpc.020040. View

14.

Gusmaroli G, Feng S, Deng X . The Arabidopsis CSN5A and CSN5B subunits are present in distinct COP9 signalosome complexes, and mutations in their JAMM domains exhibit differential dominant negative effects on development. Plant Cell. 2004; 16(11):2984-3001. PMC: 527193. DOI: 10.1105/tpc.104.025999. View

15.

Gusmaroli G, Figueroa P, Serino G, Deng X . Role of the MPN subunits in COP9 signalosome assembly and activity, and their regulatory interaction with Arabidopsis Cullin3-based E3 ligases. Plant Cell. 2007; 19(2):564-81. PMC: 1867349. DOI: 10.1105/tpc.106.047571. View

16.

Dohmann E, Levesque M, De Veylder L, Reichardt I, Jurgens G, Schmid M . The Arabidopsis COP9 signalosome is essential for G2 phase progression and genomic stability. Development. 2008; 135(11):2013-22. DOI: 10.1242/dev.020743. View

17.

Katsir L, Schilmiller A, Staswick P, He S, Howe G . COI1 is a critical component of a receptor for jasmonate and the bacterial virulence factor coronatine. Proc Natl Acad Sci U S A. 2008; 105(19):7100-5. PMC: 2383947. DOI: 10.1073/pnas.0802332105. View

18.

Wei N, Serino G, Deng X . The COP9 signalosome: more than a protease. Trends Biochem Sci. 2008; 33(12):592-600. DOI: 10.1016/j.tibs.2008.09.004. View

19.

Feiz L, Beecher B, Martin J, Giroux M . In planta mutagenesis determines the functional regions of the wheat puroindoline proteins. Genetics. 2009; 183(3):853-60. PMC: 2778981. DOI: 10.1534/genetics.109.106013. View

20.

Hind S, Pulliam S, Veronese P, Shantharaj D, Nazir A, Jacobs N . The COP9 signalosome controls jasmonic acid synthesis and plant responses to herbivory and pathogens. Plant J. 2011; 65(3):480-91. DOI: 10.1111/j.1365-313X.2010.04437.x. View