Spheroid Formation of Hepatocarcinoma Cells in Microwells: Experiments and Monte Carlo Simulations

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2016 Aug 30

PMID 27571565

Citations 9

Authors

Yan Wang

Myung Hee Kim

Seyed R Tabaei

Jae Hyeok Park

Kyuhwan Na

Seok Chung

Vladimir P Zhdanov

Nam-Joon Cho

Affiliations

Soon will be listed here.

Abstract

The formation of spherical aggregates during the growth of cell population has long been observed under various conditions. We observed the formation of such aggregates during proliferation of Huh-7.5 cells, a human hepatocarcinoma cell line, in a microfabricated low-adhesion microwell system (SpheroFilm; formed of mass-producible silicone elastomer) on the length scales up to 500 μm. The cell proliferation was also tracked with immunofluorescence staining of F-actin and cell proliferation marker Ki-67. Meanwhile, our complementary 3D Monte Carlo simulations, taking cell diffusion and division, cell-cell and cell-scaffold adhesion, and gravity into account, illustrate the role of these factors in the formation of spheroids. Taken together, our experimental and simulation results provide an integrative view of the process of spheroid formation for Huh-7.5 cells.

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