Molecular Fingerprinting Delineates Progenitor Populations in the Developing Zebrafish Enteric Nervous System

Overview

Journal Dev Dyn

Publisher Wiley

Specialty Anatomy & Histology

Date 2016 Aug 28

PMID 27565577

Citations 17

Authors

Charlotte R Taylor

William A Montagne

Judith S Eisen

Julia Ganz

Affiliations

Soon will be listed here.

Abstract

Background: To understand the basis of nervous system development, we must learn how multipotent progenitors generate diverse neuronal and glial lineages. We addressed this issue in the zebrafish enteric nervous system (ENS), a complex neuronal and glial network that regulates essential intestinal functions. Little is currently known about how ENS progenitor subpopulations generate enteric neuronal and glial diversity.

Results: We identified temporally and spatially dependent progenitor subpopulations based on coexpression of three genes essential for normal ENS development: phox2bb, sox10, and ret. Our data suggest that combinatorial expression of these genes delineates three major ENS progenitor subpopulations, (1) phox2bb + /ret- /sox10-, (2) phox2bb + /ret + /sox10-, and (3) phox2bb + /ret + /sox10+, that reflect temporal progression of progenitor maturation during migration. We also found that differentiating zebrafish neurons maintain phox2bb and ret expression, and lose sox10 expression.

Conclusions: Our data show that zebrafish enteric progenitors constitute a heterogeneous population at both early and late stages of ENS development and suggest that marker gene expression is indicative of a progenitor's fate. We propose that a progenitor's expression profile reveals its developmental state: "younger" wave front progenitors express all three genes, whereas more mature progenitors behind the wave front selectively lose sox10 and/or ret expression, which may indicate developmental restriction. Developmental Dynamics 245:1081-1096, 2016. © 2016 Wiley Periodicals, Inc.

Citing Articles

BMP signaling pathway member expression is enriched in enteric neural progenitors and required for zebrafish enteric nervous system development.

Moore J, Moreno-Campos R, Noah A, Singleton E, Uribe R Dev Dyn. 2024; 254(3):272-287.

PMID: 39297504 PMC: 11879768. DOI: 10.1002/dvdy.737.

A targeted CRISPR-Cas9 mediated F0 screen identifies genes involved in establishment of the enteric nervous system.

Moreno-Campos R, Singleton E, Uribe R PLoS One. 2024; 19(5):e0303914.

PMID: 38809858 PMC: 11135701. DOI: 10.1371/journal.pone.0303914.

A targeted CRISPR-Cas9 mediated F0 screen identifies genes involved in establishment of the enteric nervous system.

Moreno-Campos R, Singleton E, Uribe R bioRxiv. 2024; .

PMID: 38234831 PMC: 10793464. DOI: 10.1101/2023.12.28.573581.

Genetic regulation of enteric nervous system development in zebrafish.

Uribe R Biochem Soc Trans. 2024; 52(1):177-190.

PMID: 38174765 PMC: 10903509. DOI: 10.1042/BST20230343.

Who's talking to whom: microbiome-enteric nervous system interactions in early life.

Ganz J, Ratcliffe E Am J Physiol Gastrointest Liver Physiol. 2023; 324(3):G196-G206.

PMID: 36625480 PMC: 9988524. DOI: 10.1152/ajpgi.00166.2022.

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