Comparative Proteomic Analysis of Buffalo Oocytes Matured in Vitro Using ITRAQ Technique

Overview

Journal Sci Rep

Specialty Science

Date 2016 Aug 27

PMID 27561356

Citations 8

Authors

Lingsheng Chen

Linhui Zhai

Chunfeng Qu

Chengpu Zhang

Sheng Li

Feilin Wu

Yingzi Qi

Fenghua Lu

Ping Xu

Xiangping Li

Deshun Shi

Affiliations

Soon will be listed here.

Abstract

To investigate the protein profiling of buffalo oocytes at the germinal vesicle (GV) stage and metaphase II (MII) stage, an iTRAQ-based strategy was applied. A total of 3,763 proteins were identified, which representing the largest buffalo oocytes proteome dataset to date. Among these proteins identified, 173 proteins were differentially expressed in GV oocytes and competent MII oocytes, and 146 proteins were differentially abundant in competent and incompetent matured oocytes. Functional and KEGG pathway analysis revealed that the up-regulated proteins in competent MII oocytes were related to chromosome segregation, microtubule-based process, protein transport, oxidation reduction, ribosome, and oxidative phosphorylation, etc., in comparison with GV and incompetent MII oocytes. This is the first proteomic report on buffalo oocytes from different maturation stages and developmental competent status. These data will provide valuable information for understanding the molecular mechanism underlying buffalo oocyte maturation, and these proteins may potentially act as markers to predict developmental competence of buffalo oocyte during in vitro maturation.

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