Tissue- and Time-Specific Expression of Otherwise Identical TRNA Genes

Overview

Journal PLoS Genet

Specialty Genetics

Date 2016 Aug 26

PMID 27560950

Citations 39

Authors

Dror Sagi

Roni Rak

Hila Gingold

Idan Adir

Gadi Maayan

Orna Dahan

Limor Broday

Yitzhak Pilpel

Oded Rechavi

Affiliations

Soon will be listed here.

Abstract

Codon usage bias affects protein translation because tRNAs that recognize synonymous codons differ in their abundance. Although the current dogma states that tRNA expression is exclusively regulated by intrinsic control elements (A- and B-box sequences), we revealed, using a reporter that monitors the levels of individual tRNA genes in Caenorhabditis elegans, that eight tryptophan tRNA genes, 100% identical in sequence, are expressed in different tissues and change their expression dynamically. Furthermore, the expression levels of the sup-7 tRNA gene at day 6 were found to predict the animal's lifespan. We discovered that the expression of tRNAs that reside within introns of protein-coding genes is affected by the host gene's promoter. Pairing between specific Pol II genes and the tRNAs that are contained in their introns is most likely adaptive, since a genome-wide analysis revealed that the presence of specific intronic tRNAs within specific orthologous genes is conserved across Caenorhabditis species.

Citing Articles

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