Induction of Renal Senescence Marker Protein-30 (SMP30) Expression by Testosterone and Its Contribution to Urinary Calcium Absorption in Male Rats

Overview

Journal Sci Rep

Specialty Science

Date 2016 Aug 25

PMID 27553527

Citations 7

Authors

Po-Han Lin

Cai-Yun Jian

Jou-Chun Chou

Chien-Wei Chen

Chih-Chieh Chen

Christina Soong

Sindy Hu

Fu-Kong Lieu

Paulus S Wang

Shyi-Wu Wang

Affiliations

Soon will be listed here.

Abstract

The aim of this study was to investigate the involvement of androgen, mainly testosterone, in the expression of renal senescence marker protein-30 (SMP30) in male rats. We found that the renal SMP30 expression was up-regulated by endogenous testosterone stimulation during puberty. Interestingly, androgen-deficient orchidectomized (ORX) rats exhibited lower SMP30 mRNA and protein expression in the kidney, and that was restored by testosterone propionate (TP) replacement. Abrogation of androgen receptor (AR) activity by co-treatment with flutamide abolished testosterone-induced SMP30 expression in the kidney as well as in the NRK52E cells. However, SMP30 expression was unaltered in the liver of ORX rats. We also showed a positive correlation between renal SMP30 expression and plasma testosterone level during the aging process. TP-induced SMP30 expression in ovariectomized (OVX) rats was observed and was an evidence to explain the gender difference of SMP30 levels. Immunofluorescence assay showed that renal SMP30 was specifically expressed in the proximal tubular segments of the kidney. The urinary Ca(2+) level was increased in both ORX and male aging rats. Taken together, our results indicate a novel role of testosterone in regulating SMP30 expression specifically in the kidney to contribute to urinary calcium absorption.

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