BMERB Domains Are Bivalent Rab8 Family Effectors Evolved by Gene Duplication

Overview

Journal Elife

Specialty Biology

Date 2016 Aug 24

PMID 27552051

Citations 36

Authors

Amrita Rai

Anastasia Oprisko

Jeremy Campos

Yangxue Fu

Timon Friese

Aymelt Itzen

Roger S Goody

Emerich Mihai Gazdag

Matthias P Muller

Affiliations

Soon will be listed here.

Abstract

In their active GTP-bound form, Rab proteins interact with proteins termed effector molecules. In this study, we have thoroughly characterized a Rab effector domain that is present in proteins of the Mical and EHBP families, both known to act in endosomal trafficking. Within our study, we show that these effectors display a preference for Rab8 family proteins (Rab8, 10, 13 and 15) and that some of the effector domains can bind two Rab proteins via separate binding sites. Structural analysis allowed us to explain the specificity towards Rab8 family members and the presence of two similar Rab binding sites that must have evolved via gene duplication. This study is the first to thoroughly characterize a Rab effector protein that contains two separate Rab binding sites within a single domain, allowing Micals and EHBPs to bind two Rabs simultaneously, thus suggesting previously unknown functions of these effector molecules in endosomal trafficking.

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