Fatty Acid Extract from CLA-enriched Egg Yolks Can Mediate Transcriptome Reprogramming of MCF-7 Cancer Cells to Prevent Their Growth and Proliferation

Overview

Journal Genes Nutr

Publisher Biomed Central

Date 2016 Aug 24

PMID 27551323

Citations 3

Authors

Aneta A Koronowicz

Paula Banks

Dominik Domagala

Adam Master

Teresa Leszczynska

Ewelina Piasna

Mariola Marynowska

Piotr Laidler

Affiliations

Soon will be listed here.

Abstract

Background: Our previous study showed that fatty acids extract obtained from CLA-enriched egg yolks (EFA-CLA) suppressed the viability of MCF-7 cancer cell line more effectively than extract from non-enriched egg yolks (EFA). In this study, we analysed the effect of EFA-CLA and EFA on transcriptome profile of MCF-7 cells by applying the whole Human Genome Microarray technology.

Results: We found that EFA-CLA and EFA treated cells differentially regulated genes involved in cancer development and progression. EFA-CLA, compared to EFA, positively increased the mRNA expression of TSC2 and PTEN tumor suppressors as well as decreased the expression of NOTCH1, AGPS, GNA12, STAT3, UCP2, HIGD2A, HIF1A, PPKAR1A oncogenes.

Conclusions: We show for the first time that EFA-CLA can regulate genes engaged in AKT/mTOR pathway and inhibiting cell cycle progression. The observed results are most likely achieved by the combined effect of both: incorporated CLA isomers and other fatty acids in eggs organically modified through hens' diet. Our results suggest that CLA-enriched eggs could be easily available food products with a potential of a cancer chemopreventive agent.

Citing Articles

Biological Effect of Food for Special Medical Purposes (Nutramil Complex) on Melanoma Cells in In Vitro Study.

Koronowicz A, Krawczyk K, Such A, Piasna-Slupecka E, Drozdowska M, Leszczynska T Nutrients. 2025; 16(24.

PMID: 39770908 PMC: 11679902. DOI: 10.3390/nu16244287.

Mechanisms of Anticancer Activity of a Fatty Acid Mixture Extracted from Hen Egg Yolks Enriched in Conjugated Linoleic Acid Diene (CLA) against WM793 Melanoma Cells.

Domagala D, Leszczynska T, Koronowicz A, Domagala B, Drozdowska M, Piasna-Slupecka E Nutrients. 2021; 13(7).

PMID: 34371857 PMC: 8308778. DOI: 10.3390/nu13072348.

Fatty Acids of CLA-Enriched Egg Yolks Can Induce Transcriptional Activation of Peroxisome Proliferator-Activated Receptors in MCF-7 Breast Cancer Cells.

Koronowicz A, Banks P, Master A, Domagala D, Piasna-Slupecka E, Drozdowska M PPAR Res. 2017; 2017:2865283.

PMID: 28458685 PMC: 5385215. DOI: 10.1155/2017/2865283.

Erratum to: Fatty acid extract from CLA-enriched egg yolks can mediate transcriptome reprogramming of MCF-7 cancer cells to prevent their growth and proliferation.

Koronowicz A, Banks P, Domagala D, Master A, Leszczynska T, Piasna E Genes Nutr. 2016; 11:24.

PMID: 27552345 PMC: 4988027. DOI: 10.1186/s12263-016-0540-4.

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